Disease relevance of TMEFF1

• Interestingly, ectopic expression of TMEFF1 in brain cancer cells resulted in their growth inhibition [1].
• Our results also suggested that ST14 and TMEFF1 were co-expressed in the human breast cancer cell line MCF7, human placenta, kidney, and liver tissues [2].

High impact information on TMEFF1

• Finally, homology modeling studies suggested that TMEFF1 might form a complex with ST14 by an interaction between epidermal growth factor and CUB domains [2].
• With the same method, a CUB domain-interacting protein was isolated and turned out to be the transmembrane protein with epidermal growth factor-like and two follistatin-like domains 1 (TMEFF1) [2].
• Study of cancer cell lines from different tissues including the brain, demonstrated moderate to low levels of TMEFF1 in most of these transformed cell lines [1].
• These data suggest that TMEFF1 may behave as a tumor suppressor gene in brain cancers [1].
• TMEFF1 fused to enhanced green fluorescent protein revealed that TMEFF1 is expressed on the cell membrane [1].

Biological context of TMEFF1

• Quantitative real time PCR revealed that the expression of the TMEFF1 gene was dependent on the transfection of the ST14 gene in the RKO cell line [2].
• Assignment1 of H7365 (C9orf2) to human chromosome band 9q31 by somatic cell hybrid analysis and fluorescence in situ hybridization [3].

Other interactions of TMEFF1

• Interestingly, these two genes were co-up-regulated in kidney tumors versus normal tissues, consistent with our results that showed the dependence of TMEFF1 expression on ST14 in RKO cells [2].
• Furthermore, no recombination was observed between Grey, TGFBR1 and TMEFF1, the last two being 1.4 Mb apart in human [4].

Analytical, diagnostic and therapeutic context of TMEFF1

• Furthermore, quantitative real-time RT-PCR analysis of 54 brain tumors showed that most of these tumors (96%) had lower levels of TMEFF1 expression than normal brain tissue [1].

References