Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Chemical Compound Review:

AGN-PC-00BJC5 3-phenylpropanenitrile

Synonyms: SureCN181108, ACMC-1B3P0, ANW-34909, NSC-16936, AC1L1ZWF, ...

Vorwerk, S. et al., Wajant, H. et al.

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High impact information on Benzenepropanenitrile

While all nitrilases exhibit a similar pH optimum around neutral, and NIT1 and NIT3 exhibit a similar temperature optimum around 30 degrees C independent of the substrate analyzed (IAN, PPN), NIT2 showed a remarkably different temperature optimum for IAN (15 degrees C) and PPN (35-40 degrees C) [1].
In comparison with 3-indolylacetonitrile, the rate of hydrolysis of 3-phenylpropionitrile is increased by a factor of 330, and the Km value is reduced by a factor of 23 [2].

References

Enzymatic characterization of the recombinant Arabidopsis thaliana nitrilase subfamily encoded by the NIT2/NIT1/NIT3-gene cluster. Vorwerk, S., Biernacki, S., Hillebrand, H., Janzik, I., Müller, A., Weiler, E.W., Piotrowski, M. Planta (2001) [Pubmed]
Characterization and synthetic applications of recombinant AtNIT1 from Arabidopsis thaliana. Wajant, H., Effenberger, F. Eur. J. Biochem. (2002) [Pubmed]

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