High impact information on 1-FD-AA

• After hydrolysis of the silk proteins, the corresponding amino acids were derivatized with Nalpha-(2,4-dinitro-5-fluorophenyl)-L-alaninamide (Marfey's reagent) and separated by liquid chromatography [1].
• Enantiomeric determination of amphetamine and methamphetamine in urine by precolumn derivatization with Marfey's reagent and HPLC [2].
• Micellar electrokinetic capillary chromatography was used to separate levodopa (L-3,4-dihydroxyphenyl-L-alanine), dextrodopa (D-3,4-dihydroxyphenyl-L-alanine) and DL-3-O-methyl-dopa, a metabolite of dopa, which were derivatised with the Marfey's reagent, in sodium borate, sodium dodecyl sulfate and acetonitile buffer system [3].

Biological context of 1-FD-AA

• Peptide chiral purity determination: hydrolysis in deuterated acid, derivatization with Marfey's reagent and analysis using high-performance liquid chromatography-electrospray ionization-mass spectrometry [4].

Analytical, diagnostic and therapeutic context of 1-FD-AA

• The stereochemistry of the isolated acid was determined to be (R) by coelution on HPLC of its derivative with Marfey's reagent and that of an authentic sample (7) obtained by enantiospecific synthesis [5].
• Reversed-phase high-performance liquid chromatography and pre-column derivatization with 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (Marfey's reagent) were used for monitoring racemization in peptides, amino acids and their derivatives by separation of optical isomers of amino acids [6].
• Separation of amino acid enantiomers and peptide isomers has been made possible through the use of Marfey's reagent and high-performance capillary electrophoresis (HPCE) [7].
• Separation of DL-dopa by means of micellar electrokinetic capillary chromatography after derivatization with Marfey's reagent [3].

References