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MeSH Review

Electrophoresis, Capillary

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  • To determine the effect of selective alteration of CD44 oligosaccharide composition on the affinity of CD44 for HA, we developed a novel strategy based on the use of affinity capillary electrophoresis (ACE) [8].
  • Here, we report methodology, based on matrix-assisted laser desorption ionization MS and capillary electrophoresis, to follow the time course of the enzymatic degradation of heparin-like glycosaminoglycans through the intermediate stages to the end products [9].
  • Picomole amounts of oligodeoxynucleotides [polydeoxyadenylic acids, (dA)40-60] were baseline resolved and analyzed in less than 8 min by high-performance capillary electrophoresis with polyacrylamide gels [10].
  • Similarly, nitrate concentration (by capillary electrophoresis) in the media in which the rings were incubated was reduced in the hypoxic group (5.6+/-0.23 micromol/L for hypoxic rats and 7.8+/-0.7 micromol/L for normoxic rats) [11].
  • Initial analysis using high-performance capillary electrophoresis and immunolocalization revealed a loss of genomic 5-methylcytosine associated with the degree of tumor aggressiveness [12].

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  1. VLDL receptor fragments of different lengths bind to human rhinovirus HRV2 with different stoichiometry. An analysis of virus-receptor complexes by capillary electrophoresis. Okun, V.M., Moser, R., Ronacher, B., Kenndler, E., Blaas, D. J. Biol. Chem. (2001) [Pubmed]
  2. Prostate-specific membrane antigen: a novel folate hydrolase in human prostatic carcinoma cells. Pinto, J.T., Suffoletto, B.P., Berzin, T.M., Qiao, C.H., Lin, S., Tong, W.P., May, F., Mukherjee, B., Heston, W.D. Clin. Cancer Res. (1996) [Pubmed]
  3. Purification of the Escherichia coli K5 capsular polysaccharide and use of high-performance capillary electrophoresis to qualitative and quantitative monitor the process. Volpi, N. Electrophoresis (2004) [Pubmed]
  4. Investigation of the potential of capillary electrophoresis with off-line matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for clinical analysis: examination of a glycoprotein factor associated with cancer cachexia. Choudhary, G., Chakel, J., Hancock, W., Torres-Duarte, A., McMahon, G., Wainer, I. Anal. Chem. (1999) [Pubmed]
  5. Analysis of a polyadenine tract of the transforming growth factor-beta type II receptor gene in colorectal cancers by non-gel-sieving capillary electrophoresis. Oto, M., Koguchi, A., Yuasa, Y. Clin. Chem. (1997) [Pubmed]
  6. Nanoscale liquid chromatography and capillary electrophoresis coupled to electrospray mass spectrometry for the detection of amyloid-beta peptide related to Alzheimer's disease. Varesio, E., Rudaz, S., Krause, K.H., Veuthey, J.L. Journal of chromatography. A. (2002) [Pubmed]
  7. Carbohydrate-deficient transferrin determination revisited with capillary electrophoresis: a new biochemical marker of chronic alcohol abuse. Tagliaro, F., Bortolotti, F., Zuliani, M., Crivellente, F., Manett, G., Pascali, V.L., Marigo, M. Journal of capillary electrophoresis. (1999) [Pubmed]
  8. Glycosylation provides both stimulatory and inhibitory effects on cell surface and soluble CD44 binding to hyaluronan. Skelton, T.P., Zeng, C., Nocks, A., Stamenkovic, I. J. Cell Biol. (1998) [Pubmed]
  9. Mass spectrometric and capillary electrophoretic investigation of the enzymatic degradation of heparin-like glycosaminoglycans. Rhomberg, A.J., Ernst, S., Sasisekharan, R., Biemann, K. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  10. Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis. Cohen, A.S., Najarian, D.R., Paulus, A., Guttman, A., Smith, J.A., Karger, B.L. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  11. Downregulation of endothelial nitric oxide synthase in rat aorta after prolonged hypoxia in vivo. Toporsian, M., Govindaraju, K., Nagi, M., Eidelman, D., Thibault, G., Ward, M.E. Circ. Res. (2000) [Pubmed]
  12. A mouse skin multistage carcinogenesis model reflects the aberrant DNA methylation patterns of human tumors. Fraga, M.F., Herranz, M., Espada, J., Ballestar, E., Paz, M.F., Ropero, S., Erkek, E., Bozdogan, O., Peinado, H., Niveleau, A., Mao, J.H., Balmain, A., Cano, A., Esteller, M. Cancer Res. (2004) [Pubmed]
  13. Characterization of thioredoxins by sodium dodecyl sulfate-slab gel electrophoresis and high performance capillary electrophoresis. Bunik, V., Shoubnikova, A., Bisswanger, H., Follmann, H. Electrophoresis (1997) [Pubmed]
  14. Biomonitoring of urinary tamoxifen and its metabolites from breast cancer patients using nonaqueous capillary electrophoresis with electrospray mass spectrometry. Carter, S.J., Li, X.F., Mackey, J.R., Modi, S., Hanson, J., Dovichi, N.J. Electrophoresis (2001) [Pubmed]
  15. Separation of Escherichia coli 055:B5 lipopolysaccharide and detoxified lipopolysaccharide by high-performance capillary electrophoresis. Volpi, N. Electrophoresis (2003) [Pubmed]
  16. Penetration of capecitabine and its metabolites into malignant and healthy tissues of patients with advanced breast cancer. Mader, R.M., Schrolnberger, C., Rizovski, B., Brunner, M., Wenzel, C., Locker, G., Eichler, H.G., Mueller, M., Steger, G.G. Br. J. Cancer (2003) [Pubmed]
  17. Identification of hyaluronic acid oligosaccharides by direct coupling of capillary electrophoresis with electrospray ion trap mass spectrometry. Kühn, A.V., Rüttinger, H.H., Neubert, R.H., Raith, K. Rapid Commun. Mass Spectrom. (2003) [Pubmed]
  18. Strategies for nonradioactive methods in the localization of phosphorylated amino acids in proteins. Meyer, H.E., Eisermann, B., Heber, M., Hoffmann-Posorske, E., Korte, H., Weigt, C., Wegner, A., Hutton, T., Donella-Deana, A., Perich, J.W. FASEB J. (1993) [Pubmed]
  19. High-precision genotyping by denaturing capillary electrophoresis. Wenz, H., Robertson, J.M., Menchen, S., Oaks, F., Demorest, D.M., Scheibler, D., Rosenblum, B.B., Wike, C., Gilbert, D.A., Efcavitch, J.W. Genome Res. (1998) [Pubmed]
  20. Substrate specificities of three members of the human UDP-N-acetyl-alpha-D-galactosamine:Polypeptide N-acetylgalactosaminyltransferase family, GalNAc-T1, -T2, and -T3. Wandall, H.H., Hassan, H., Mirgorodskaya, E., Kristensen, A.K., Roepstorff, P., Bennett, E.P., Nielsen, P.A., Hollingsworth, M.A., Burchell, J., Taylor-Papadimitriou, J., Clausen, H. J. Biol. Chem. (1997) [Pubmed]
  21. Ultradian rapid cycling in prepubertal and early adolescent bipolarity is not in transmission disequilibrium with val/met COMT alleles. Geller, B., Cook, E.H. Biol. Psychiatry (2000) [Pubmed]
  22. Sample matrix effects on glycopeptide stability by high performance capillary electrophoresis. Rush, R.S., Boss, H.J., Katta, V., Rohde, M.F. Electrophoresis (1997) [Pubmed]
  23. Central nervous system nitric oxide formation in cerebral systemic lupus erythematosus. Brundin, L., Svenungsson, E., Morcos, E., Andersson, M., Olsson, T., Lundberg, I., Wiklund, N.P. Ann. Neurol. (1998) [Pubmed]
  24. Amphetamine redistributes dopamine from synaptic vesicles to the cytosol and promotes reverse transport. Sulzer, D., Chen, T.K., Lau, Y.Y., Kristensen, H., Rayport, S., Ewing, A. J. Neurosci. (1995) [Pubmed]
  25. Separation of amino acid homopolymers by capillary gel electrophoresis. Dolník, V., Novotny, M.V. Anal. Chem. (1993) [Pubmed]
  26. Analysis of carbonic anhydrase in human red Blood cells using capillary electrophoresis/ electrospray ionization-mass spectrometry. Moini, M., Demars, S.M., Huang, H. Anal. Chem. (2002) [Pubmed]
  27. Measurement of alkaline phosphatase isoenzymes in individual mouse bone marrow fibroblast cells based on capillary electrophoresis with on-capillary enzyme-catalyzed reaction and electrochemical detection. Sun, X., Jin, W., Li, D., Bai, Z. Electrophoresis (2004) [Pubmed]
  28. Serotonin catabolism and the formation and fate of 5-hydroxyindole thiazolidine carboxylic Acid. Squires, L.N., Jakubowski, J.A., Stuart, J.N., Rubakhin, S.S., Hatcher, N.G., Kim, W.S., Chen, K., Shih, J.C., Seif, I., Sweedler, J.V. J. Biol. Chem. (2006) [Pubmed]
  29. Real-time detection of basal and stimulated G protein GTPase activity using fluorescent GTP analogues. Jameson, E.E., Roof, R.A., Whorton, M.R., Mosberg, H.I., Sunahara, R.K., Neubig, R.R., Kennedy, R.T. J. Biol. Chem. (2005) [Pubmed]
  30. UDP-N-acetylglucosamine pyrophosphorylase, a key enzyme in encysting Giardia, is allosterically regulated. Bulik, D.A., van Ophem, P., Manning, J.M., Shen, Z., Newburg, D.S., Jarroll, E.L. J. Biol. Chem. (2000) [Pubmed]
  31. Expression, purification, and biochemical characterization of WbpP, a new UDP-GlcNAc C4 epimerase from Pseudomonas aeruginosa serotype O6. Creuzenet, C., Belanger, M., Wakarchuk, W.W., Lam, J.S. J. Biol. Chem. (2000) [Pubmed]
  32. Conformational intermediate of the amyloidogenic protein beta 2-microglobulin at neutral pH. Heegaard, N.H., Sen, J.W., Kaarsholm, N.C., Nissen, M.H. J. Biol. Chem. (2001) [Pubmed]
  33. Determination of hereditary mutations in the BRCA1 gene using archived serum samples and capillary electrophoresis. Ekstrøm, P.O., Bjørge, T., Dørum, A., Longva, A.S., Heintz, K.M., Warren, D.J., Hansen, S., Gislefoss, R.E., Hovig, E. Anal. Chem. (2004) [Pubmed]
  34. Capillary electrophoresis in diagnosis and monitoring of adenosine deaminase deficiency. Carlucci, F., Tabucchi, A., Aiuti, A., Rosi, F., Floccari, F., Pagani, R., Marinello, E. Clin. Chem. (2003) [Pubmed]
  35. BRAF mutation detection and identification by cycling temperature capillary electrophoresis. Hinselwood, D.C., Abrahamsen, T.W., Ekstrøm, P.O. Electrophoresis (2005) [Pubmed]
  36. Genotype-phenotype correlation of mouse pde6b mutations. Hart, A.W., McKie, L., Morgan, J.E., Gautier, P., West, K., Jackson, I.J., Cross, S.H. Invest. Ophthalmol. Vis. Sci. (2005) [Pubmed]
  37. Single-strand conformation polymorphism analysis by capillary and microchip electrophoresis: a fast, simple method for detection of common mutations in BRCA1 and BRCA2. Tian, H., Jaquins-Gerstl, A., Munro, N., Trucco, M., Brody, L.C., Landers, J.P. Genomics (2000) [Pubmed]
  38. Identification of constitutive and gamma-interferon- and interleukin 4-regulated proteins in the human renal carcinoma cell line ACHN. Sullivan, C.M., Smith, D.M., Matsui, N.M., Andrews, L.E., Clauser, K.R., Chapeaurouge, A., Burlingame, A.L., Epstein, L.B. Cancer Res. (1997) [Pubmed]
  39. The mutational spectrum of the HPRT gene from human T cells in vivo shares a significant concordant set of hot spots with MNNG-treated human cells. Tomita-Mitchell, A., Ling, L.L., Glover, C.L., Goodluck-Griffith, J., Thilly, W.G. Cancer Res. (2003) [Pubmed]
  40. Separation and analysis of DNA by electrophoresis. Smith, C.L. Curr. Opin. Biotechnol. (1991) [Pubmed]
  41. Microchip-based capillary electrophoresis for immunoassays: analysis of monoclonal antibodies and theophylline. Chiem, N., Harrison, D.J. Anal. Chem. (1997) [Pubmed]
  42. Felbamate measured in serum by two methods: HPLC and capillary electrophoresis. Shihabi, Z.K., Oles, K.S. Clin. Chem. (1994) [Pubmed]
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