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Chemical Compound Review:

AG-G-51446 5-[(1S,2S,5S)-7-oxo-3-thia- 6,8...

Synonyms: B7639_SIGMA, Probes2_000011, AC1Q6FUC, CTK2F5802, ZINC02839654, ...

Aragón, V. et al., Brimer, C.D. et al., Kähne, T. et al., Yoo, B.S. et al., Massó, F. et al.

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Disease relevance of ZINC02839654

Identification of major glycoconjugates from Mycobacterium bovis culture filtrate by biotin-hydrazide labeling [1].

High impact information on ZINC02839654

Biotinylation of yeast protein was accomplished during extraction at 4 degrees C in a lysis buffer containing 5 mM biotin-hydrazide [2].
A biotin-hydrazide glycosylation assay was performed on the flagellins of three a-type strains (170,018, 5933, and 5939) and one b-type strain (M2), revealing a positive glycosylation reaction for strains 5933 and 5939 and a negative reaction for 170,018 and M2 [3].
CD3 and CD26 immunoreactivity was diminished using NHS biotinylation but preserved by biotin-LC-hydrazide, whereas CD65 binding to monoclonal antibodies was completely abolished after treatment with biotin-hydrazide [4].

Analytical, diagnostic and therapeutic context of ZINC02839654

First, NH became biotinylated when B. abortus live cells were labelled with biotin-hydrazide, and the examination of cell fractions and electron microscopy sections with streptavidin-peroxidase and streptavidin-coloidal gold, respectively, showed that labelling was extrinsic [5].

References

Identification of major glycoconjugates from Mycobacterium bovis culture filtrate by biotin-hydrazide labeling. Massó, F., Varela, E., Páez, A., Zenteno, E., Montaño, L.F. Glycoconj. J. (1998) [Pubmed]
Proteomic analysis of carbonylated proteins in two-dimensional gel electrophoresis using avidin-fluorescein affinity staining. Yoo, B.S., Regnier, F.E. Electrophoresis (2004) [Pubmed]
Cloning and comparison of fliC genes and identification of glycosylation in the flagellin of Pseudomonas aeruginosa a-type strains. Brimer, C.D., Montie, T.C. J. Bacteriol. (1998) [Pubmed]
Non-radioactive labelling and immunoprecipitation analysis of leukocyte surface proteins using different methods of protein biotinylation. Kähne, T., Ansorge, S. J. Immunol. Methods (1994) [Pubmed]
Characterization of Brucella abortus and Brucella melitensis native haptens as outer membrane O-type polysaccharides independent from the smooth lipopolysaccharide. Aragón, V., Díaz, R., Moreno, E., Moriyón, I. J. Bacteriol. (1996) [Pubmed]

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