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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Gene Review: 

cyp  -  cytochrome P460

Nitrosomonas europaea ATCC 19718

 
 
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Disease relevance of cyp

  • Gene probing using cyp indicated that a cytochrome P460 similar to that from M. capsulatus Bath may be present in the type II methanotrophs Methylosinus trichosporium OB3b and Methylocystis parvus OBBP but not in the type I methanotrophs Methylobacter marinus A45, Methylomicrobium albus BG8, and Methylomonas sp. strains MN and MM2 [1].
  • The gene for this protein, cyp, was expressed in Pseudomonas aeruginosa strain PAO lacI, resulting in formation of a holo-cytochrome P460 which closely resembled native cytochrome P460 purified from N. europaea in its UV-visible spectroscopic, ligand binding and catalytic properties [2].

References

  1. Cytochrome P460 genes from the methanotroph Methylococcus capsulatus bath. Bergmann, D.J., Zahn, J.A., Hooper, A.B., DiSpirito, A.A. J. Bacteriol. (1998) [Pubmed]
  2. Cytochrome P460 of Nitrosomonas europaea. Formation of the heme-lysine cross-link in a heterologous host and mutagenic conversion to a non-cross-linked cytochrome c'. Bergmann, D.J., Hooper, A.B. Eur. J. Biochem. (2003) [Pubmed]
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