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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Gene Review: 

nifD  -  nitrogenase molybdenum-iron protein...

Geobacter sulfurreducens PCA

 
 
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Disease relevance of nifD

  • When Geobacter sulfurreducens was grown without a source of fixed nitrogen in chemostats with acetate provided as the limiting electron donor and Fe(III) as the electron acceptor, levels of nifD transcripts were 4 to 5 orders of magnitude higher than in chemostat cultures provided with ammonium [1].
 

High impact information on nifD

  • Levels of nifD transcripts in Geobacteraceae decreased more than 100-fold within 2 days after the addition of 100 microM ammonium, while levels of recA and total bacterial 16S rRNA in Geobacteraceae remained relatively constant [1].
  • In order to determine whether the metabolic state of Geobacteraceae involved in bioremediation of subsurface sediments might be inferred from levels of mRNA for key genes, in situ expression of nifD, a highly conserved gene involved in nitrogen fixation, was investigated [1].
  • Expression of the nifD gene in trans complemented this phenotype [2].
 

Associations of nifD with chemical compounds

  • The addition of acetate to organic- and nitrogen-poor subsurface sediments stimulated the growth of Geobacteraceae and Fe(III) reduction, as well as the expression of nifD in Geobacteraceae [1].

References

  1. In situ expression of nifD in Geobacteraceae in subsurface sediments. Holmes, D.E., Nevin, K.P., Lovley, D.R. Appl. Environ. Microbiol. (2004) [Pubmed]
  2. Development of a genetic system for Geobacter sulfurreducens. Coppi, M.V., Leang, C., Sandler, S.J., Lovley, D.R. Appl. Environ. Microbiol. (2001) [Pubmed]
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