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Gene Review:

Mbar_A0843 - monomethylamine:corrinoid methyltransferase

Methanosarcina barkeri str. Fusaro

Hao, B. et al., Polycarpo, C. et al., Longstaff, D.G. et al., Burke, S.A. et al.

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High impact information on mtmB

Monomethylamine methyltransferase of the archaeon Methanosarcina barkeri contains a rare amino acid, pyrrolysine, encoded by the termination codon UAG [1].
Host mtmB expression was minimized by growth on methanol and recombinant mtmB1 products monitored by anti-MtmB and anti-hexahistidine immunoblotting [2].
L-pyrrolysine, the 22(nd) genetically encoded amino acid, was previously deduced to be (4R, 5R)-4-substituted-pyrroline-5-carboxylate attached to the epsilon-nitrogen of lysine based on the crystal structure of the M. barkeri monomethylamine methyltransferase (MtmB) [3].
The open reading frame of mtmB was interrupted by a single in-frame, midframe, UAG codon which was also found in mtmB from M. barkeri NIH [4].

References

Activation of the pyrrolysine suppressor tRNA requires formation of a ternary complex with class I and class II lysyl-tRNA synthetases. Polycarpo, C., Ambrogelly, A., Ruan, B., Tumbula-Hansen, D., Ataide, S.F., Ishitani, R., Yokoyama, S., Nureki, O., Ibba, M., Söll, D. Mol. Cell (2003) [Pubmed]
In vivo contextual requirements for UAG translation as pyrrolysine. Longstaff, D.G., Blight, S.K., Zhang, L., Green-Church, K.B., Krzycki, J.A. Mol. Microbiol. (2007) [Pubmed]
Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acid. Hao, B., Zhao, G., Kang, P.T., Soares, J.A., Ferguson, T.K., Gallucci, J., Krzycki, J.A., Chan, M.K. Chem. Biol. (2004) [Pubmed]
Clustered genes encoding the methyltransferases of methanogenesis from monomethylamine. Burke, S.A., Lo, S.L., Krzycki, J.A. J. Bacteriol. (1998) [Pubmed]

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