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Gene Review:

ccdB - plasmid maintenance protein CcdB

Escherichia coli

Umanskaya, O.N. et al., Chen, Q.J. et al.

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Disease relevance of ccdB

Each entry vector contains an AhdI cassette flanked by attL sites, with each AhdI cassette containing two AhdI restriction enzyme sites spaced by the ccdB killer gene, which is lethal to most Escherichia coli strains [1].

High impact information on ccdB

The system is based on a plasmid construct containing an inducible marker gene ccdB ("killer" (KIL) gene) whose product is lethal for bacterial cells, flanked by two different potentially recombinogenic elements [2].
The plasmids were subjected to recombinogenic conditions in vitro or in vivo after transient transfection into COS-1 cells, and subsequently transformed into E. coli which was then grown in the presence of the ccdB gene inducer [2].

References

Using a modified TA cloning method to create entry clones. Chen, Q.J., Zhou, H.M., Chen, J., Wang, X.C. Anal. Biochem. (2006) [Pubmed]
Inhibition of DNA topoisomerase II may trigger illegitimate recombination in living cells: Experiments with a model system. Umanskaya, O.N., Lebedeva, S.S., Gavrilov, A.A., Bystritskiy, A.A., Razin, S.V. J. Cell. Biochem. (2006) [Pubmed]

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