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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

COS Cells

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Disease relevance of COS Cells

  • These cell lines (ts COS cells) produce high levels of thermolabile large T antigen under the transcriptional control of the Rous sarcoma virus long terminal repeat [1].
  • CD59 antigen was released from the surface of transfected COS cells by phosphatidylinositol-specific phospholipase C, demonstrating that it is attached to the cell membrane by means of a glycolipid anchor; it is therefore likely to be absent from the surface of affected erythrocytes in the disease paroxysmal nocturnal hemoglobinuria [2].
  • A series of deletion mutants constructed from a full-length cDNA clone of the Simian 11 rotavirus VP7 gene were expressed in COS 7 cells [3].
  • Furthermore, HXB-3 proviral clones of HIV-1 containing either a wild-type or mutated version of the nef gene replicated in an indistinguishable manner when transfected into COS cells [4].
  • The function of the viral proteinase is also essential for secretion of flaviviral spike proteins when expressed from cDNA via vaccinia virus recombinants or in COS cell transfections [5].

Psychiatry related information on COS Cells

  • When APP(SW) (Swedish familial Alzheimer's disease mutation), a preferable substrate for BACE1, was coexpressed with ST6Gal I in COS cells, the secretion of ST6Gal I significantly decreased, suggesting that that the beta-cleavage of overexpressed APP(SW) competes with ST6Gal I processing [6].

High impact information on COS Cells

  • This procedure involves coexpressing an FKBP-tagged Golgi enzyme with an ER-retained protein fused to FRAP in COS cells [7].
  • When expressed in COS-7 cells, Cys226 and Met49 variants had diminished and aberrant activity, respectively, in interconverting isomers of retinol and retinal [8].
  • Co-transfection of Nkx2-5 and Tbx5 into COS-7 cells showed that they also associate with each other in mammalian cells [9].
  • In two clones of COS-7 cells transfected with the mutant sequence from one patient, the sensitivity of the enzyme to guanosine 5'-triphosphate was reduced, findings similar to those in the child's lymphoblasts [10].
  • To elucidate the molecular mechanisms whereby expanded polyglutamine stretches elicit a gain of toxic function, we expressed full-length and truncated DRPLA (dentatorubral-pallidoluysian atrophy) cDNAs with or without expanded CAG repeats in COS-7 cells [11].

Chemical compound and disease context of COS Cells


Biological context of COS Cells


Anatomical context of COS Cells


Associations of COS Cells with chemical compounds

  • Both 239nef and YEnef were found to associate with src in cotransfected COS cells, and both 60 kDa src and 34 kDa nef were phosphorylated at tyrosine in these cells [27].
  • A soluble ligand construct, when coexpressed with fucosyltransferase in COS cells, also mediates P-selectin binding and is immunocrossreactive with the major HL-60 glycoprotein that specifically binds P-selectin [28].
  • Expression of AE3 cDNA in COS cells leads to chronic cytoplasmic acidification and to chloride- and bicarbonate-dependent changes in intracellular pH, confirming that this gene product is an anion exchanger [29].
  • Expression of this gene in COS cells resulted in a heat-labile arylsulfatase activity that is inhibited by warfarin [30].
  • An increase in Ca2+, phosphatidylserine, and diacylglycerol/phorbol-ester-dependent protein kinase activity is also observed in COS cells transfected with either PKC-I or PKC-II [31].

Gene context of COS Cells

  • The cloned cDNA, when transfected into COS cells, leads to overexpression of an approximately 80 kd protein that specifically binds radioiodinated TGF-beta 1 [32].
  • Expression of mutant IPF1 in Cos-1 cells confirms the expression of a prematurely terminated truncated protein of 16 kD [33].
  • We report here the isolation of a complementary DNA clone encoding CD16 determinants which gave rise to IgG binding of the expected affinity and subtype specificity in COS cells, and which proved to encode a phospholipid anchored protein [34].
  • Overexpression of PAIP-1 in COS-7 cells stimulates translation, perhaps by providing a physical link between the mRNA termini [35].
  • When expressed in monkey COS cells, the daf-4 receptor binds human BMP-2 and BMP-4 [36].

Analytical, diagnostic and therapeutic context of COS Cells


  1. A mammalian host-vector system that regulates expression and amplification of transfected genes by temperature induction. Rio, D.C., Clark, S.G., Tjian, R. Science (1985) [Pubmed]
  2. CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. Davies, A., Simmons, D.L., Hale, G., Harrison, R.A., Tighe, H., Lachmann, P.J., Waldmann, H. J. Exp. Med. (1989) [Pubmed]
  3. Deletions into an NH2-terminal hydrophobic domain result in secretion of rotavirus VP7, a resident endoplasmic reticulum membrane glycoprotein. Poruchynsky, M.S., Tyndall, C., Both, G.W., Sato, F., Bellamy, A.R., Atkinson, P.H. J. Cell Biol. (1985) [Pubmed]
  4. Nef protein of human immunodeficiency virus type 1: evidence against its role as a transcriptional inhibitor. Hammes, S.R., Dixon, E.P., Malim, M.H., Cullen, B.R., Greene, W.C. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  5. Flavivirus premembrane protein cleavage and spike heterodimer secretion require the function of the viral proteinase NS3. Lobigs, M. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  6. Alzheimer's beta-secretase, beta-site amyloid precursor protein-cleaving enzyme, is responsible for cleavage secretion of a Golgi-resident sialyltransferase. Kitazume, S., Tachida, Y., Oka, R., Shirotani, K., Saido, T.C., Hashimoto, Y. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  7. Golgi membranes remain segregated from the endoplasmic reticulum during mitosis in mammalian cells. Pecot, M.Y., Malhotra, V. Cell (2004) [Pubmed]
  8. Mutations in RDH12 encoding a photoreceptor cell retinol dehydrogenase cause childhood-onset severe retinal dystrophy. Janecke, A.R., Thompson, D.A., Utermann, G., Becker, C., Hübner, C.A., Schmid, E., McHenry, C.L., Nair, A.R., Rüschendorf, F., Heckenlively, J., Wissinger, B., Nürnberg, P., Gal, A. Nat. Genet. (2004) [Pubmed]
  9. Tbx5 associates with Nkx2-5 and synergistically promotes cardiomyocyte differentiation. Hiroi, Y., Kudoh, S., Monzen, K., Ikeda, Y., Yazaki, Y., Nagai, R., Komuro, I. Nat. Genet. (2001) [Pubmed]
  10. Hyperinsulinism and hyperammonemia in infants with regulatory mutations of the glutamate dehydrogenase gene. Stanley, C.A., Lieu, Y.K., Hsu, B.Y., Burlina, A.B., Greenberg, C.R., Hopwood, N.J., Perlman, K., Rich, B.H., Zammarchi, E., Poncz, M. N. Engl. J. Med. (1998) [Pubmed]
  11. Suppression of aggregate formation and apoptosis by transglutaminase inhibitors in cells expressing truncated DRPLA protein with an expanded polyglutamine stretch. Igarashi, S., Koide, R., Shimohata, T., Yamada, M., Hayashi, Y., Takano, H., Date, H., Oyake, M., Sato, T., Sato, A., Egawa, S., Ikeuchi, T., Tanaka, H., Nakano, R., Tanaka, K., Hozumi, I., Inuzuka, T., Takahashi, H., Tsuji, S. Nat. Genet. (1998) [Pubmed]
  12. Identification of semaphorin E as a non-MDR drug resistance gene of human cancers. Yamada, T., Endo, R., Gotoh, M., Hirohashi, S. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  13. Tetracycline-regulated suppression of amber codons in mammalian cells. Park, H.J., RajBhandary, U.L. Mol. Cell. Biol. (1998) [Pubmed]
  14. Parkinsonism-inducing neurotoxin MPP+: uptake and toxicity in nonneuronal COS cells expressing dopamine transporter cDNA. Kitayama, S., Shimada, S., Uhl, G.R. Ann. Neurol. (1992) [Pubmed]
  15. Mutation of alanine 623 in the third cytoplasmic loop of the rat thyrotropin (TSH) receptor results in a loss in the phosphoinositide but not cAMP signal induced by TSH and receptor autoantibodies. Kosugi, S., Okajima, F., Ban, T., Hidaka, A., Shenker, A., Kohn, L.D. J. Biol. Chem. (1992) [Pubmed]
  16. Expression of the phospholipid-dependent Escherichia coli sn-1,2-diacylglycerol kinase in COS cells perturbs cellular lipid composition. Ramer, J.K., Bell, R.M. J. Biol. Chem. (1990) [Pubmed]
  17. Identification of a common mutation in the carnitine palmitoyltransferase II gene in familial recurrent myoglobinuria patients. Taroni, F., Verderio, E., Dworzak, F., Willems, P.J., Cavadini, P., DiDonato, S. Nat. Genet. (1993) [Pubmed]
  18. Defective intracellular transport and processing of CFTR is the molecular basis of most cystic fibrosis. Cheng, S.H., Gregory, R.J., Marshall, J., Paul, S., Souza, D.W., White, G.A., O'Riordan, C.R., Smith, A.E. Cell (1990) [Pubmed]
  19. Activation of the SV40 late promoter: direct effects of T antigen in the absence of viral DNA replication. Keller, J.M., Alwine, J.C. Cell (1984) [Pubmed]
  20. Adult-onset spinocerebellar dysfunction caused by a mutation in the gene for the alpha-tocopherol-transfer protein. Gotoda, T., Arita, M., Arai, H., Inoue, K., Yokota, T., Fukuo, Y., Yazaki, Y., Yamada, N. N. Engl. J. Med. (1995) [Pubmed]
  21. A second molecular form of D2 dopamine receptor in rat and bovine caudate nucleus. Chio, C.L., Hess, G.F., Graham, R.S., Huff, R.M. Nature (1990) [Pubmed]
  22. Redox regulation of a protein tyrosine kinase in the endoplasmic reticulum. Bauskin, A.R., Alkalay, I., Ben-Neriah, Y. Cell (1991) [Pubmed]
  23. CD36 directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Oquendo, P., Hundt, E., Lawler, J., Seed, B. Cell (1989) [Pubmed]
  24. PADGEM-dependent adhesion of platelets to monocytes and neutrophils is mediated by a lineage-specific carbohydrate, LNF III (CD15). Larsen, E., Palabrica, T., Sajer, S., Gilbert, G.E., Wagner, D.D., Furie, B.C., Furie, B. Cell (1990) [Pubmed]
  25. Spatio-temporal images of growth-factor-induced activation of Ras and Rap1. Mochizuki, N., Yamashita, S., Kurokawa, K., Ohba, Y., Nagai, T., Miyawaki, A., Matsuda, M. Nature (2001) [Pubmed]
  26. Ataxin-1 with an expanded glutamine tract alters nuclear matrix-associated structures. Skinner, P.J., Koshy, B.T., Cummings, C.J., Klement, I.A., Helin, K., Servadio, A., Zoghbi, H.Y., Orr, H.T. Nature (1997) [Pubmed]
  27. Identification of a nef allele that causes lymphocyte activation and acute disease in macaque monkeys. Du, Z., Lang, S.M., Sasseville, V.G., Lackner, A.A., Ilyinskii, P.O., Daniel, M.D., Jung, J.U., Desrosiers, R.C. Cell (1995) [Pubmed]
  28. Expression cloning of a functional glycoprotein ligand for P-selectin. Sako, D., Chang, X.J., Barone, K.M., Vachino, G., White, H.M., Shaw, G., Veldman, G.M., Bean, K.M., Ahern, T.J., Furie, B. Cell (1993) [Pubmed]
  29. Regulation of intracellular pH by a neuronal homolog of the erythrocyte anion exchanger. Kopito, R.R., Lee, B.S., Simmons, D.M., Lindsey, A.E., Morgans, C.W., Schneider, K. Cell (1989) [Pubmed]
  30. A cluster of sulfatase genes on Xp22.3: mutations in chondrodysplasia punctata (CDPX) and implications for warfarin embryopathy. Franco, B., Meroni, G., Parenti, G., Levilliers, J., Bernard, L., Gebbia, M., Cox, L., Maroteaux, P., Sheffield, L., Rappold, G.A. Cell (1995) [Pubmed]
  31. Cloning and expression of multiple protein kinase C cDNAs. Knopf, J.L., Lee, M.H., Sultzman, L.A., Kriz, R.W., Loomis, C.R., Hewick, R.M., Bell, R.M. Cell (1986) [Pubmed]
  32. Expression cloning of the TGF-beta type II receptor, a functional transmembrane serine/threonine kinase. Lin, H.Y., Wang, X.F., Ng-Eaton, E., Weinberg, R.A., Lodish, H.F. Cell (1992) [Pubmed]
  33. Pancreatic agenesis attributable to a single nucleotide deletion in the human IPF1 gene coding sequence. Stoffers, D.A., Zinkin, N.T., Stanojevic, V., Clarke, W.L., Habener, J.F. Nat. Genet. (1997) [Pubmed]
  34. The Fc gamma receptor of natural killer cells is a phospholipid-linked membrane protein. Simmons, D., Seed, B. Nature (1988) [Pubmed]
  35. Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation. Craig, A.W., Haghighat, A., Yu, A.T., Sonenberg, N. Nature (1998) [Pubmed]
  36. The daf-4 gene encodes a bone morphogenetic protein receptor controlling C. elegans dauer larva development. Estevez, M., Attisano, L., Wrana, J.L., Albert, P.S., Massagué, J., Riddle, D.L. Nature (1993) [Pubmed]
  37. SH3GL3 associates with the Huntingtin exon 1 protein and promotes the formation of polygln-containing protein aggregates. Sittler, A., Wälter, S., Wedemeyer, N., Hasenbank, R., Scherzinger, E., Eickhoff, H., Bates, G.P., Lehrach, H., Wanker, E.E. Mol. Cell (1998) [Pubmed]
  38. Cloning and functional characteristics of murine large granular lymphocyte-1: a member of the Ly-49 gene family (Ly-49G2). Mason, L.H., Ortaldo, J.R., Young, H.A., Kumar, V., Bennett, M., Anderson, S.K. J. Exp. Med. (1995) [Pubmed]
  39. Unique C1 inhibitor dysfunction in a kindred without angioedema. II. Identification of an Ala443-->Val substitution and functional analysis of the recombinant mutant protein. Zahedi, R., Bissler, J.J., Davis, A.E., Andreadis, C., Wisnieski, J.J. J. Clin. Invest. (1995) [Pubmed]
  40. The axonal recognition molecule F11 is a multifunctional protein: specific domains mediate interactions with Ng-CAM and restrictin. Brümmendorf, T., Hubert, M., Treubert, U., Leuschner, R., Tárnok, A., Rathjen, F.G. Neuron (1993) [Pubmed]
  41. MuSK is required for anchoring acetylcholinesterase at the neuromuscular junction. Cartaud, A., Strochlic, L., Guerra, M., Blanchard, B., Lambergeon, M., Krejci, E., Cartaud, J., Legay, C. J. Cell Biol. (2004) [Pubmed]
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