High impact information on PCL9

• In addition, we found that expression of one member of the Pcl1,2 subfamily, PCL9, is cell cycle regulated and is decreased in cells arrested in G1 by pheromone treatment [1].
• This gene, PCL9, is indeed regulated by Swi5 in late M phase, the only cyclin known to be expressed at this point in the cell cycle [2].
• The Pcl9 protein is associated with a Pho85-dependent protein kinase activity, and the protein is unstable with peak levels occurring in late M phase [2].
• Results:. To identify targets for Pcl-Pho85 kinases, we performed yeast two-hybrid interaction screens using Pcl2 and the related cyclin Pcl9 [3].
• Deletion of PCL9 in diploid cells caused random, rather than bipolar, budding in 18% of cells [4].

Enzymatic interactions of PCL9

• Furthermore, immunoprecipitated Pcl9 complexes from yeast lysates were capable of phosphorylating the exogenous substrate Pho4 [4].

Other interactions of PCL9

• Our results show that PCL9 and PHO85 form a functional kinase complex and suggest a role for Pho85 CDKs at the M/G1 boundary [4].
• In contrast, deletion of PCL2, the closest relative of PCL9, had no effect on the budding pattern [4].

Analytical, diagnostic and therapeutic context of PCL9

• Co-immunoprecipitation experiments using in vitro translated proteins showed that Pcl9 and Pho85 form a complex [4].

References