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Gene Review:

CEF1 - Cef1p

Saccharomyces cerevisiae S288c

Synonyms: NTC85, PRP nineteen-associated complex protein 85, PRP19-associated complex protein 85, Pre-mRNA-splicing factor CEF1, YM8261.07, ...

Ohi, M.D. et al., Ohi, R. et al., Ohi, M.D. et al., Burns, C.G. et al., Burns, C.G. et al., et al.

Burns, Ohi, Krainer, Gould,

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High impact information on CEF1

Furthermore, genetic depletion of the homolog of CDC5 in Saccharomyces cerevisiae, CEF1, blocks the first step of pre-mRNA processing in vivo [1].
Here we provide evidence that cell cycle arrest in a new temperature-sensitive CEF1 mutant, cef1-13, is an indirect consequence of defects in pre-mRNA splicing [2].
Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs [3].
Although it is possible that Cef1p acts as a transcriptional activator, we have demonstrated that Cef1p is not involved in transcriptional activation of a class of G2/M-regulated genes typified by SWI5 [4].
Cef1p was associated with the spliceosome in the same manner as Prp19p, i.e. concomitant with or immediately after dissociation of U4 [5].

Anatomical context of CEF1

In S. cerevisiae, a subcomplex of the spliceosome that includes Cef1p can be isolated on its own; this has been termed the nineteen complex (Ntc) because it contains Prp19p [6].

Physical interactions of CEF1

At least 26 proteins were detected in the Cdc5p/Cef1p complexes [3].

Other interactions of CEF1

Two-hybrid analysis revealed that none of these proteins interacted with Prp19p or Ntc25p, but all interacted with Ntc85p [7].

References

Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing. Burns, C.G., Ohi, R., Krainer, A.R., Gould, K.L. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae. Burns, C.G., Ohi, R., Mehta, S., O'Toole, E.T., Winey, M., Clark, T.A., Sugnet, C.W., Ares, M., Gould, K.L. Mol. Cell. Biol. (2002) [Pubmed]
Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Ohi, M.D., Link, A.J., Ren, L., Jennings, J.L., McDonald, W.H., Gould, K.L. Mol. Cell. Biol. (2002) [Pubmed]
Myb-related Schizosaccharomyces pombe cdc5p is structurally and functionally conserved in eukaryotes. Ohi, R., Feoktistova, A., McCann, S., Valentine, V., Look, A.T., Lipsick, J.S., Gould, K.L. Mol. Cell. Biol. (1998) [Pubmed]
Cef1p is a component of the Prp19p-associated complex and essential for pre-mRNA splicing. Tsai, W.Y., Chow, Y.T., Chen, H.R., Huang, K.T., Hong, R.I., Jan, S.P., Kuo, N.Y., Tsao, T.Y., Chen, C.H., Cheng, S.C. J. Biol. Chem. (1999) [Pubmed]
Characterization of interactions among the Cef1p-Prp19p-associated splicing complex. Ohi, M.D., Gould, K.L. RNA (2002) [Pubmed]
Functional and physical interactions between components of the Prp19p-associated complex. Chen, C.H., Yu, W.C., Tsao, T.Y., Wang, L.Y., Chen, H.R., Lin, J.Y., Tsai, W.Y., Cheng, S.C. Nucleic Acids Res. (2002) [Pubmed]

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