High impact information on CROCC

• Moreover, rootletin interacts with C-Nap1, a protein previously implicated in centrosome cohesion [1].
• Similar to C-Nap1, rootletin is phosphorylated by Nek2 kinase and is displaced from centrosomes at the onset of mitosis [1].
• In this study, we analyze the protein rootletin as a candidate centrosome linker component [1].
• As shown by immunoelectron microscopy, endogenous rootletin forms striking fibers emanating from the proximal ends of centrioles [1].

Anatomical context of CROCC

• Recombinant rootletin forms detergent-insoluble filaments radiating from the centrioles and resembling rootlets found in vivo [2].

Other interactions of CROCC

• Whereas the overexpression of rootletin results in the formation of extensive fibers, small interfering RNA-mediated depletion of either rootletin or C-Nap1 causes centrosome splitting, suggesting that both proteins contribute to maintaining centrosome cohesion [1].

References