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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Purification of supercoiled plasmid DNA using chromatographic processes.

The interest in purifying injectable-grade plasmid DNA has increased with the development of gene therapy and DNA vaccination technologies. In this paper we develop a method for purifying a 4.8 kb plasmid based on chromatographic processes. An NaCl gradient was optimized on a Q Sepharose column and plasmid was eluted at 800-820 mM NaCl in a broad peak. Supercoiled plasmid was isolated after a final Sepharcryl S1000 SF gel filtration step. Final plasmid preparation was depleted of proteins and RNA, as revealed by the BCA assay and 1% agarose gel electrophoresis.[1]


  1. Purification of supercoiled plasmid DNA using chromatographic processes. Ferreira, G.N., Cabral, J.M., Prazeres, D.M. J. Mol. Recognit. (1998) [Pubmed]
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