Accumulation of poly (3-hydroxybutyric acid) by some soil Streptomyces.
In a limited-scale survey, 55 soil streptomycetes were screened for the accumulation of poly (3-hydroxybutyrate) [PHB]. Only 18% of the isolates accumulated PHB ranging between 1.9-7.8% of the dry biomass. The promising isolate DBCC-719, identified as Streptomyces griseorubiginosus, accumulated PHB amounting to 9.5% of the mycelial dry mass in the early stationary phase when grown in chemically defined medium with 2% (wt/vol) glucose as the sole source of carbon. Nitrogen-limiting conditions were inhibitory to growth and PHB accumulation. The isolated polymer was highly soluble in chloroform, gave a sharp peak at 235 nm on digestion with concentrated H(2)SO(4), and had a characteristic infrared spectrum.[1]References
- Accumulation of poly (3-hydroxybutyric acid) by some soil Streptomyces. Manna, A., Banerjee, R., Paul, A.K. Curr. Microbiol. (1999) [Pubmed]
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