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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A reversed-phase high-performance liquid chromatographic method to analyze retinal isomers.

A high-performance liquid chromatographic (HPLC) procedure was developed to separate all-trans-, 13-cis-, 11-cis- and 9-cis-retinal isomers. Two reversed-phase Vydac C18 columns in series were used with an isocratic solvent system of 0.1 M ammonium acetate-acetonitrile (40:60, v/v) as mobile phase and all-trans-9-(4-methoxy-2,3,6-trimethylphenyl)-3,7-dimethyl-2,4,6,8-no natetraene-1-ol (TMMP) as internal standard. Prior to HPLC, the retinal isomers were efficiently extracted in their original isomeric conformation using dichloromethane-n-hexane in the presence of formaldehyde. This technique is suitable for the assay of 11-cis- and all-trans-retinal isomers in retina.[1]

References

  1. A reversed-phase high-performance liquid chromatographic method to analyze retinal isomers. Cia, D., Bonhomme, B., Azim, M., Wada, A., Doly, M., Azaïs-Braesco, W. Journal of chromatography. A. (1999) [Pubmed]
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