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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A rapid method for PCR detection of bovine materials in animal feedstuffs.

Rapid identification of bovine materials in animal feedstuffs is essential for effective control of a potential source of bovine spongiform encephalopathy. We have developed a rapid method for the detection of the presence of bovine materials in animal feeds. Animal feed samples were prepared by a Chelex-100 treatment method, then subjected to polymerase chain reaction (PCR) detection. The assay can be completed in 2 h including 30 min for sample preparation, 35-65 min for PCR cycling and 30 min for gel electrophoresis. This method is not only rapid, simple and consistent, but also avoids a hazardous waste disposal issue associated with a previously described guanidine thiocyanate (GuSCN) extraction-PCR method.[1]

References

  1. A rapid method for PCR detection of bovine materials in animal feedstuffs. Wang, R.F., Myers, M.J., Campbell, W., Cao, W.W., Paine, D., Cerniglia, C.E. Mol. Cell. Probes (2000) [Pubmed]
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