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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Freezing induces artificial cleavage of apoptosis-related proteins in human bone marrow cells.

The aim of this study was to investigate whether freeze-thawing of freshly isolated human mononuclear bone marrow cells (MNC) influences the integrity of apoptosis-related proteins as determined by immunoblot analyses. Our results show that bone marrow is more sensitive to this process than either myelomonocytoid leukemic P39 or Jurkat T-lymphocyte cell lines. Specifically, bone marrow cells displayed a high level of intrinsic proteolytic activity in response to a single freeze-thaw cycle, which led to the cleavage of various proteins involved in apoptosis cell signaling. This effect was completely blocked by the inclusion of broad-spectrum protease inhibitors in the freezing medium and subsequently thawing the cells on ice. Since differences in the freezing conditions (-80 degrees C vs. liquid nitrogen) did not alter the proteins of interest, we suggest that the thawing process is the critical point when proteolytic enzyme activity is elevated.[1]

References

  1. Freezing induces artificial cleavage of apoptosis-related proteins in human bone marrow cells. Schmidt-Mende, J., Hellström-Lindberg, E., Joseph, B., Zhivotovsky, B. J. Immunol. Methods (2000) [Pubmed]
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