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Kcc4 associates with septin proteins of Saccharomyces cerevisiae.

Kcc4, a kinase of the budding yeast Saccharomyces cerevisiae, is homologous to the bud neck protein kinases Hsl1/Nik1 and Gin4. We report here that a GFP-Kcc4 fusion protein is localized at the bud neck and that the non-kinase domain is required for this localization. We also demonstrate that Kcc4 associates with septin proteins in vitro and in vivo by two-hybrid analysis, GST pull-down experiments, immunoprecipitation, and analysis of direct association with affinity-purified GST-Kcc4 and MBP-Septin proteins. From the results obtained here, we suggest that Cdc11 is the primary association partner of Kcc4.[1]


  1. Kcc4 associates with septin proteins of Saccharomyces cerevisiae. Okuzaki, D., Nojima, H. FEBS Lett. (2001) [Pubmed]
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