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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Primary culture of chicken hepatocytes as an in vitro model for determining the influence of dioxin.

An easy method for primary culture of chicken hepatocytes was developed to study the influence of dioxin on birds. Chicken hepatocytes could maintain gene expression and protein secretion of albumin for a long period in serum-free medium with free atmosphere exchange at 37 degrees C. Moreover, the cells showed a sensitive response to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) by monitoring the expression of P450 1A, theta GST (theta-GST) and albumin genes.[1]

References

  1. Primary culture of chicken hepatocytes as an in vitro model for determining the influence of dioxin. Hou, D.X., Kunitake, T., Kusuda, J., Fujii, M. Biosci. Biotechnol. Biochem. (2001) [Pubmed]
 
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