The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Editor

Share

Personal info

View

Links

About

Terms

 

 
 

Resonance Rayleigh method for the determination of proteins with Orange G.

In pH 0.6 - 2.0 HCl-sodium acetate buffer solution, proteins react with an acidic monoazo dye such as Orange G, Methyl Orange, Methyl Red and Orange IV to form a combination product. This results in a significant enhancement of resonance Rayleigh scattering (RRS) and a new RRS spectrum appears. Owing to the fact that Orange G-protein system is the most sensitive, it was taken as an example to study. The RRS spectral characteristics of its combination product and the optimum condition for the reaction were investigated. The intensity of RRS is directly proportional to the concentration of protein in the range of 0 - 5.0 microg/mL. The method has high sensitivity; its detection limits are 2.6 ng/mL for BSA, 3.4 ng/mL for HAS and 7.1 ng/mL for alpha-chymotrypsin, respectively. A new method for the determination of trace amounts of proteins on the basis of RRS spectra has been developed.[1]

References

  1. Resonance Rayleigh method for the determination of proteins with Orange G. Liu, S., Yang, R., Liu, Q. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry. (2001) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
 
WikiGenes - Universities