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High-performance liquid chromatographic determination of ambroxol in human plasma.

Ambroxol has been determined in biological fluids using a rapid and sensitive high-performance liquid chromatographic method. The samples prepared from plasma by liquid-liquid extraction were analysed on reversed-phase silica gel by competing-ion chromatography with ultraviolet detection. The method was applied to the determination of ambroxol levels in twelve healthy volunteers after oral administration of 90 mg of ambroxol in tablets of Mucosolvan and Ambrosan.[1]


  1. High-performance liquid chromatographic determination of ambroxol in human plasma. Nobilis, M., Pastera, J., Svoboda, D., Kvêtina, J., Macek, K. J. Chromatogr. (1992) [Pubmed]
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