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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Circular dichroism imaging microscopy: application to enantiomorphous twinning in biaxial crystals of 1,8-dihydroxyanthraquinone.

A microscope was constructed for imaging circular dichroism of heterogeneous anisotropic media. To avoid linear biases that are common with electronic circular polarization modulation, we chose a retrogressive solution: mechanical light modulation by rotating a linear polarizer with respect to a quarter wave plate continuously tuned by tilting to the operating wavelength. Our comparatively slow technique succeeds with near-perfect circular input and signal averaging using a CCD camera. We have applied the method to anomalously birefringent crystals of 1,8-dihydroxyanthraquinone that are shown to have intergrown mirror image domains, undetected by X-ray diffraction because the twinning complexity renders differences in anomalous dispersion, already small, unreliable. The origin of the anomalous birefringence and the assignment of the absolute configuration are discussed.[1]

References

  1. Circular dichroism imaging microscopy: application to enantiomorphous twinning in biaxial crystals of 1,8-dihydroxyanthraquinone. Claborn, K., Puklin-Faucher, E., Kurimoto, M., Kaminsky, W., Kahr, B. J. Am. Chem. Soc. (2003) [Pubmed]
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