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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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In vivo transfer of the ti-plasmid of Agrobacterium tumefaciens to Escherichia coli.

The Ti-plasmids are naturally self-transmissible from their normal host Agrobacterium to E. coli. They are however unable to stably establish themselves as a replicon in E. coli. It is nevertheless possible to study the Ti-plasmids in E. coli with the help of Ti::RP4 cointegrate plasmids that transfer and maintain themselves very efficiently in E. coli. An E. coli harbouring such a Ti::RP4 plasmid is unable to catabolize octopine and unable to induce crown-gall tumours on plants.[1]

References

  1. In vivo transfer of the ti-plasmid of Agrobacterium tumefaciens to Escherichia coli. Holsters, M., Silva, B., Van Vliet, F., Hernalsteens, J.P., Genetello, C., Van Montagu, M., Schell, J. Mol. Gen. Genet. (1978) [Pubmed]
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