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Johnson, J.R. et al.

Johnson, Owens, Sabate, Prats,

Rapid and specific detection of the O15:K52:H1 clonal group of Escherichia coli by gene-specific PCR.

Primers specific for Escherichia coli O15:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC, i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the O15:K52:H1 clonal group, including 162 diverse clinical and reference E. coli isolates.[1]

References

Rapid and specific detection of the O15:K52:H1 clonal group of Escherichia coli by gene-specific PCR. Johnson, J.R., Owens, K., Sabate, M., Prats, G. J. Clin. Microbiol. (2004) [Pubmed]

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