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Ikeda, M. et al.

Pre-B-cell colony formation assay.

Latent membrane protein 2A (LMP-2A) of Epstein-Barr virus (EBV) mimics a constitutively active B-cell receptor (BCR) and plays a key role in viral latency and EBV pathogenesis. By functioning as a BCR mimic, LMP-2A drives B-cell development, resulting in the bypass of normal B-cell developmental checkpoints. To assess the function of LMP-2A, we have utilized a colony formation assay for the progenitor B cells that uses the B-cell proliferation factor IL-7.[1]

References

Pre-B-cell colony formation assay. Ikeda, M., Longnecker, R. Methods Mol. Biol. (2005) [Pubmed]

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