The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Effects of alkylating metabolites of ifosfamide and its bromo analogues on DNA of HeLa cells.

The in vitro cytotoxicity and mechanism of action of three alkylating compounds: an active metabolite of ifosfamide (1, isophosphoramide mustard, N,N'-bis(2-chloroethyl)phosphorodiamidic acid) and its bromo substituted analogues, 2 (one chlorine atom replaced by bromine atom) and 3 (two chlorine atoms replaced by bromine atoms), were studied in cultured HeLa cells. Alkaline elution analysis of cellular DNA demonstrated the presence of concentration- and time-dependent interstrand crosslinks, DNA-protein crosslinks and alkali-labile sites (ALSs) in HeLa cells following a 1 hr exposure to the compounds. The bromo analogues were more cytotoxic than 1 and exhibited higher crosslinking potency. The time-course of crosslink formation and removal for the three compounds was similar. ALSs in DNA were produced by all tested drugs but 3 exhibited exceptionally high activity and was able to induce two kinds of alkali-labile lesion (fast- and slow-appearing) whereas 1 and 2 generated only slow-appearing ones. The results suggest that 1 and 2 are more specific in their reaction with DNA in that they produced a lesser variety of lesions than 3. A potential advantage of 2 over 1 seems to be its higher DNA interstrand crosslinking activity.[1]

References

  1. Effects of alkylating metabolites of ifosfamide and its bromo analogues on DNA of HeLa cells. Studzian, K., Kinas, R., Ciesielska, E., Szmigiero, L. Biochem. Pharmacol. (1992) [Pubmed]
 
WikiGenes - Universities