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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Determination of 4-amino-m-cresol and 5-amino-o-cresol by high performance liquid chromatography and fluorescence derivatization using fluorescamine.

For the determination of two oxidation hair dyes, 4-amino-m-cresol (4-AC) and 5-amino-o-cresol (5-AC), a sensitive isocratic high performance liquid chromatography (HPLC) method using the reversed phase mode was developed. The hair dyes were pre-column derivatized with fluorescamine prior to injection. Sensitivity could be improved 10-fold for 4-AC and 50-fold for 5-AC by fluorescence detection compared to UV detection. The limit of detection was 1 ng/injection for 4-AC and 100 pg/injection for 5-AC, respectively. For the determination of both compounds in aqueous biological matrices in order to simulate conditions for penetration studies with pig skin, a solid phase extraction procedure using C18 cartridges and acetonitrile (ACN) for elution could be developed. Average recovery was 83.4% with a coefficient of variation (CV) of 2.64% for intra-day assay and 3.20% for inter-day assay for 5-AC and 2.89% and 3.41% for 4-AC, respectively.[1]

References

  1. Determination of 4-amino-m-cresol and 5-amino-o-cresol by high performance liquid chromatography and fluorescence derivatization using fluorescamine. Eggenreich, K., Zach, E., Beck, H., Wintersteiger, R. J. Biochem. Biophys. Methods (2004) [Pubmed]
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