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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Protein kinase Akt/ PKB phosphorylates heme oxygenase-1 in vitro and in vivo.

Heme oxygenase-1 ( HO-1) is a stress response protein that protects cells against diverse noxious stimuli. Although regulation of HO-1 occurs mainly at the transcriptional level, its posttranslational modifications remain unexplored. We have identified a putative consensus sequence for phosphorylation by Akt/ PKB of HO-1 at Ser188. Recombinant human and rat HO-1, but not mutant HO-1(S188A), are phosphorylated in vitro by Akt/ PKB. Isotopic 32P-labeling of HEK293T cells confirmed that HO-1 is a phosphoprotein and that the basal HO-1 phosphorylation is increased by Akt1 activation. HO-1(S188D), a single point mutant equivalent to the phosphorylated protein, exhibited over 1.6-fold higher activity than wild type HO-1. Fluorescence resonance energy transfer (FRET) studies indicated that HO-1(S188D) bound to cytochrome P450 reductase (CPR) and biliverdin reductase (BVR) with a slightly lower Kd than wild-type HO-1. Although the changes in activity are small, this study provides the first evidence for a role of the survival kinase Akt in the regulation of HO-1.[1]


  1. Protein kinase Akt/PKB phosphorylates heme oxygenase-1 in vitro and in vivo. Salinas, M., Wang, J., Rosa de Sagarra, M., Martín, D., Rojo, A.I., Martin-Perez, J., Ortiz de Montellano, P.R., Cuadrado, A. FEBS Lett. (2004) [Pubmed]
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