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1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide interference with Lowry method.

1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was tested for interference with the Lowry method of protein determination. The EDC interference was nearly additive at both 660 and 750 nm. Blue-colored complex developed in the case of EDC and showed maximum absorbance at 750 nm, similar to the bovine serum albumin ( BSA) estimation. In time response analysis, blue-colored complex was developed after 30 min of incubation in both cases (EDC and BSA); therefore, it followed the same kinetic pattern of color development as that of BSA. Interference is believed to be caused by the reduction of the folin-ciocalteu reagent by EDC, resulting in increased blue-colored complex and apparently causing increased protein content of the sample.[1]

References

  1. 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide interference with Lowry method. Kumar, R., Shukla, A.K., Bagga, E., Kumari, S., Bajpai, R.P., Bharadwaj, L.M. Anal. Biochem. (2005) [Pubmed]
 
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