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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Synthesis of homogenous site-selectively glycosylated proteins.

Apparently homogenous glycoproteins can be synthesised in good yield by a combination of site directed mutagenesis, a highly flexible but selective chemical derivatisation and efficient purification through the use of glycosyl thiosulfonates such as 2-((biotinoyl)-amino)-ethyl methanethiosulfonate.[1]

References

  1. Synthesis of homogenous site-selectively glycosylated proteins. Swanwick, R.S., Daines, A.M., Flitsch, S.L., Allemann, R.K. Org. Biomol. Chem. (2005) [Pubmed]
 
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