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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

LC determination of citral in Cymbopogon citratus volatile oil.

It was the aim of this study to develop and validate a HPLC method for the quantitative determination of citral in Cymbopogon citratus volatile oil. The HPLC assay was performed using a Spherisorb CN column (250 mm x 4.6 mm, 5 microm), a n-hexane:ethanol (85:15) mobile phase and an UV detector (set at 233 nm). The following parameters were evaluated: linearity, precision, accuracy, specificity, quantification and detection limits. The method showed linearity in the range of 10.0-30.0 microg ml(-1). Precision and accuracy were determined at the concentration of 20 microg ml(-1). The concentration of citral in C. citratus volatile oil obtained with this assay was 75%. The HPLC method developed in this study showed an excellent performance (linearity, precision, accuracy and specificity) and can be applied to assay citral in volatile oil.[1]

References

  1. LC determination of citral in Cymbopogon citratus volatile oil. Rauber, C.d.a. .S., Guterres, S.S., Schapoval, E.E. Journal of pharmaceutical and biomedical analysis. (2005) [Pubmed]
 
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