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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Statistics of protein library construction.

SUMMARY: We have investigated the statistics associated with constructing and sampling large protein-encoding libraries. Using fairly simple statistics we have written algorithms for estimating the diversity in libraries generated by the most commonly used protocols, including error-prone PCR, DNA shuffling, StEP PCR, oligonucleotide-directed randomization, MAX randomization, synthetic shuffling, DHR, ADO and SISDC. AVAILABILITY: Web interface and C++ source code available at SUPPLEMENTARY INFORMATION: Complete mathematical notes, model assumptions and justification, users' guide and worked examples at above website.[1]


  1. Statistics of protein library construction. Firth, A.E., Patrick, W.M. Bioinformatics (2005) [Pubmed]
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