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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations.

We report a novel application for the operator-repressor titration (ORT) plasmid maintenance system. The ability of ORT to maintain a plasmid during production of DNA has been demonstrated previously. In this study, we have used the ORT system to maintain a plasmid during high cell density cultivation and expression of a recombinant protein. No evidence of plasmid loss was seen during protein expression at high cell densities. In addition, the quantity of protein produced using this system was similar to traditional plasmid maintenance systems.[1]

References

  1. Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations. Durany, O., Bassett, P., Weiss, A.M., Cranenburgh, R.M., Ferrer, P., López-Santín, J., de Mas, C., Hanak, J.A. Biotechnol. Bioeng. (2005) [Pubmed]
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