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Gioacchini, A.M. et al.

Gioacchini, Menotta, Bertini, Rossi, Zeppa, Zambonelli, Piccoli, Stocchi,

Solid-phase microextraction gas chromatography/ mass spectrometry: a new method for species identification of truffles.

This study describes a rapid method to identify different truffle species by analysis of their volatile compound fraction using static headspace solid-phase microextraction gas chromatography/ mass spectrometry. The volatile organic compounds (VOCs) were extracted using a new 2-cm 50/30 microm DVB/CAR/PDMS fiber placed for 10 min in the headspace of the truffle sample with the vial maintained at 20 degrees C (in a thermostatically controlled analysis room). The mass spectra of the VOC chromatograms were represented as 'fingerprints' of the analysed samples. Next, stepwise factorial discriminant analysis afforded a limited number of characteristic fragment ions that allowed a classification of the truffle species studied. This new method provides an effective approach to rapid quality control and identification of truffle species by analysis of their volatile fraction. Moreover, this method offers the advantage of minimizing thermal, mechanical, and chemical modifications of the truffles, thereby reducing the risk of analytical artifacts.[1]

References

Solid-phase microextraction gas chromatography/mass spectrometry: a new method for species identification of truffles. Gioacchini, A.M., Menotta, M., Bertini, L., Rossi, I., Zeppa, S., Zambonelli, A., Piccoli, G., Stocchi, V. Rapid Commun. Mass Spectrom. (2005) [Pubmed]

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