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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Application of functional group modified substrate in room-temperature phosphorescence, I--beta-cyclodextrin modified paper substrate for enrichment and determination of fluorene and acenaphthene.

A novel method for the determination of fluorene and acenaphthene on solid phase extraction-room-temperature phosphorescence (SPE-RTP) was studied. beta-cyclodextrin (beta-CD) was chemically bonded onto chromatography paper by reaction with epichorohydrin in an ultrasonic bath. The RTP signal of fluorene and acenaphthene included on the beta-CD-modified paper was increased more than 10 times compared with non-modified filter paper, indicating the formation of the inclusion complex. The conditions for the of RTP of compounds were studied in detail. The linear ranges of fluorene and acenaphthene concentrations to the RTP intensity were over two orders of magnitude (8.0 x 10(-7)-4.0 x 10(-5) mol/L for fluorene) with a correlation coefficient of 0.999. The concentration limits of detection for fluorene and acenaphthene were 1.11 x 10(-8) mol/L and 3.8 x 10(-7) mo/L, respectively. When the sampling volume was 10 microL, the absolute LODs for fluorene and acenaphthene were 18.4 pg/spot and 0.58 ng/spot, respectively. The modified filter paper was used for solid phase extraction (SPE) and the retention behaviour of fluorene and acenaphthene was examined. The enrichment efficiency of the analytes was higher than 100-fold. The SPE-RTP coupling technique was applied directly to the determination of fluorene and acenaphthnene in environmental water samples.[1]


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