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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Azaguanine-resistant mutants induced by several mutagens in a neurospora heterokaryon.

This paper reports the development of a new system for the detection of forward mutations in a heterokaryon of Neurospora crassa. This system, designed to detect a wide variety of genetic alterations, is based upon the detection of 8-azaguanine-resistant mutants in an adenine-requiring heterokaryon carrying the aza-3 gene. Induction of mutations in the aza-3 gene was detected after treatment of conidia with ultraviolet light, X-rays, ethyl methanesulfonate (EMS), or the acridine mustard, ICR-170. The pattern of appearance of mutant colonies and various factors in mutant selection are discussed.[1]

References

  1. Azaguanine-resistant mutants induced by several mutagens in a neurospora heterokaryon. Hoffmann, G.R., Malling, H.V. Mutat. Res. (1975) [Pubmed]
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