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Production, isolation and characterization of human profilin from Saccharomyces cerevisiae.

The yeast Saccharomyces cerevisiae was used to express human profilin cDNA. The recombinant protein, isolated by affinity chromatography on poly(L-proline)-Sepharose followed by ion exchange chromatography, associates with non-muscle actin and phosphatidylinositol-(4,5)-bisphosphate as authentic profilin.[1]

References

  1. Production, isolation and characterization of human profilin from Saccharomyces cerevisiae. Aspenström, P., Lassing, I., Karlsson, R. J. Muscle Res. Cell. Motil. (1991) [Pubmed]
 
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