Disease relevance of Ion Exchange

• The purified p12 phosphoprotein of Rauscher murine leukemia virus was fractionated by ion exchange chromatography into subpopulations of molecules containing different amounts of covalently linked phosphate [1].
• Recombinant human TFF1 expressed in Escherichia coli was purified by affinity chromatography, ion-exchange chromatography, and gel filtration [2].
• The 47-kD protein was partially purified from cells harvested by leukapheresis from a patient with acute myelogenous leukemia, using ammonium sulfate precipitation and ion exchange chromatography [3].
• A cyclic-nucleotide independent heparin-sensitive nuclear protein kinase (NII) from the Morris hepatoma 3924A has been purified by a combination of ion exchange and affinity chromatographic procedures and velocity gradient centrifugation [4].
• Phage polysaccharide and glycanohydrolase fractionate similarly on ion exchange resins and gel electrophoresis in sodium dodecyl sulfate, but each may be purified to homogeneity by the procedures employed [5].

Psychiatry related information on Ion Exchange

• Gamma-aminobutyric acid (GABA) was measured by the ion-exchange fluorometric method in CSF from 22 individuals at risk for Huntington's disease (HD), six individuals with HD, and five neurologically normal controls [6].

High impact information on Ion Exchange

• The processing activity was partially purified by ion exchange chromatography and sucrose gradient sedimentation [7].
• Upon fractionation of the postnuclear S100 by chromatography on ion exchange and gel filtration columns, the stimulatory activity copurifies with small ribonucleoprotein particles [8].
• After ion-exchange chromatography or gel filtration of the conditioned media and measurement of MSA by 3H-thymidine incorporation or radioreceptor assay, MSA again was found in the 3A medium but not in the 3A2 or 61t media [9].
• These remarkable dynamics of luminal [Ca2+] result from a fast and highly cooperative Ca2+/K+ ion-exchange process rather than from Ca2+ transport into the lumen [10].
• Amino acid analysis by ion exchange chromatography is just as important an analytical tool in 1986 as it was in the late 1950s, when Moore and Stein first described an automated AAA instrument [11].

Chemical compound and disease context of Ion Exchange

• Cytochrome c oxidase has been purified from the ammonia oxidizing chemoautotroph Nitrosomonas europaea by ion-exchange chromatography in the presence of Triton X-100 [12].
• We have purified an acidic octapeptide from the neural ganglion of the protochordate Ciona intestinalis by a three-step procedure including C18 Sep-Pak fractionation, MonoQ ion-exchange chromatography, and C4 reversed-phase high-performance liquid chromatography [13].
• A ubiquinol-cytochrome c oxidoreductase (cytochrome bc1) complex has been purified from the plasma membrane of aerobically grown Paracoccus denitrificans by extraction with dodecyl maltoside and ion exchange chromatography of the extract [14].
• A deoxyuridine triphosphate nucleotidohydrolase (dUTPase) which is induced in KB cells infected with herpes simplex virus type 1 (HSV-1) was purified approximately 175-fold using affinity, hydrophobic, adsorption, and ion-exchange chromatography techniques [15].
• The molybdenum-iron protein of Azotobacter vinelandii nitrogenase was separated into two subunits of equal concentration by ion exchange chromatography on sulfopropyl (SP) Sephadex at pH 5.4 in 7 M urea [16].

Biological context of Ion Exchange

• We show here that two different forms of hTFIIIB can be separated physically by ion exchange chromatography. hTFIIIB-alpha shows strong preference for transcription of the U6 over the VAI gene and does not contain TATA binding protein (TBP) [17].
• In the studies reported here, using standard biochemical procedures--i.e., ammonium sulfate fractionation, ion-exchange and gel filtration chromatography, isoelectric precipitation, and preparative polyacrylamide gel electrophoresis--we have purified a factor from human plasma that supports epidermal cell movement [18].
• TFIIIB transcriptional activity and TATA-box binding activity cofractionate during a series of four ion-exchange chromatographic steps, and reconstituted transcription reactions demonstrate that the TATA-box DNA-protein complex contains TFIIIB TAF activity [19].
• The ion-exchange behavior of the LAG-containing oligosaccharides before and after mild acid hydrolysis indicated the presence of sialic acid residues [20].
• Proteolysis of Nereis cuticle collagen by two bacterial collagenases was investigated using viscosimetry, enzyme kinetics, sodium dodecyl sulfate polyacrylamide gel electrophoresis, and ion exchange chromatography of collagenolytic peptides [21].

Anatomical context of Ion Exchange

• Macrophage stimulating protein (MSP) was purified to homogeneity from human blood plasma by selection of biologically active fractions obtained by sequential immunoaffinity and high pressure liquid ion exchange chromatography [22].
• Cultured cells were incubated in the presence of L-[2,3-3H]proline, and the collagens synthesized and secreted into the culture medium were analyzed by electrophoresis on acrylamide gels, ion exchange chromatography, and immunoprecipitation [23].
• Extracts of human colon mucosa and muscle were fractionated by gel filtration and ion exchange chromatography and molecular forms of vasoactive intestinal peptide (VIP) estimated by radioimmunoassay [24].
• Two peaks of NGF-stimulated S6 phosphotransferase activity are observed upon ion exchange chromatography; one that comigrates with the serine kinase previously described in chicken embryo fibroblasts and another with distinct elution properties [25].
• Synaptophysin, a mol. wt 38 000 glycopolypeptide of the synaptic vesicle membrane, was solubilized using Triton X-100 and purified by immunoaffinity or ion-exchange chromatography [26].

Associations of Ion Exchange with chemical compounds

• Refined methods for separating PTC-amino acids on reverse phase columns may pose a challenge to traditional ion exchange techniques [27].
• Secondary isotope effects were also observed on ion-exchange chromatography, 3H-labelled folates with 3H at the C-9 position eluting fractionally earlier than the corresponding unlabelled or [2-14C]folate from DEAE-cellulose, a behaviour similar to that reported earlier for isotopically labelled 2-aminopurine and several amino acids [28].
• Conditioned media from cells grown with or without L-triiodothyronine (T3) were treated with an ion exchange resin to remove T3 and were tested for ability to stimulate the division of GH4C1 cells [29].
• The purification of human C8 in milligram quantities from outdated human serum was achieved by ammonium sulfate precipitation (37.5-50% saturation) and ion exchange column chromatography employing CM-32 cellulose and QAE-Sephadex [30].
• Excellent correlation of the distribution of Bb protein and cell-spreading activity was found upon purification of Bb by ion-exchange and molecular seive chromatography and alkaline polyacrylamide gel electrophoresis [31].

Gene context of Ion Exchange

• Gel and ion-exchange chromatography permitted isolation of the SAA polymorphs in homogeneous form [32].
• Further fractionation with an ion-exchange chromatography revealed Le(b)-positive MUC5AC glycoforms that differed in their receptor properties for different H. pylori strains [33].
• In order to define the structure of the alpha and beta subunits generated in the lysosome, the alpha, beta a, and beta b polypeptides of hexosaminidase A and B were separated by a combination of molecular sieve and ion exchange high performance liquid chromatography, and amino-terminal sequences were determined [34].
• Ion exchange by AtNHX1 was inhibited 70% by the amiloride analog ethylisopropyl-amiloride [35].
• Herein, we show that ion exchange chromatography separates ARF from the major phospholipase D-stimulating cytosolic factor [36].

Analytical, diagnostic and therapeutic context of Ion Exchange

• The pI-6.8 species of normal human interleukin 1 (IL-1) has been isolated by ion-exchange and reverse-phase high-performance liquid chromatography [37].
• The CSPG was purified by a three-step procedure involving associative ion-exchange chromatography, immunoprecipitation, and dissociative ion-exchange chromatography [38].
• Plasma EPCR was purified from human citrated plasma using ion exchange, immunoaffinity, and protein C affinity chromatography [39].
• Moreover, characterization of MetRS activities from mitochondria and chloroplasts of pea showed that only one MetRS activity exists in each organelle and that both are indistinguishable by their behavior on ion exchange and hydrophobic chromatographies [40].
• Purification of this platelet-derived growth factor (PDGF) was accomplished by heat (100 degrees C) treatment of washed platelets and subsequent ion-exchange chromatography, gel filtration in 1 M acetic acid, isoelectric focusing, and preparative sodium dodecyl sulfate/polyacrylamide gel electrophoresis [41].

References