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Raugei, G. et al.

Chemical synthesis and expression of a gene coding for bovine liver phosphotyrosine-protein phosphatase.

A chemically synthesized polynucleotide sequence coding for bovine liver low molecular weight acid phosphatase (which possesses phosphotyrosine-protein phosphatase activity) has been cloned in a E. coli expression vector. The recombinant protein retains correct affinity for substrate and inhibitors but shows a reduced specific activity.[1]

References

Chemical synthesis and expression of a gene coding for bovine liver phosphotyrosine-protein phosphatase. Raugei, G., Marzocchini, R., Modesti, A., Ratti, G., Cappugi, G., Camici, G., Manao, G., Ramponi, G. Biochem. Int. (1991) [Pubmed]

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