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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

A simple and rapid determination of biapenem in plasma by high-performance liquid chromatography.

A simple, rapid and precise HPLC method using ultrafiltration to remove plasma protein was developed to determine biapenem concentrations in human plasma. Plasma was separated by centrifugation at 4 degrees C from blood collected in heparinized vacuum tubes, and biapenem was stabilized by immediate mixing the plasma with 1M 3-morpholinopropanesulfonic acid (MOPS) buffer (pH 7.0) (1:1). Biapenem was detected by ultraviolet absorbance at 300nm with no interfering plasma peak. The calibration curve of biapenem in human plasma was linear from 0.04 to 50mug/mL. The limit of detection was 0.01mug/mL, which was more than 40-fold lower than that of conventional plasma protein precipitation using ammonium sulfate. The assay has been clinically applied to pharmacokinetic studies in patients.[1]


  1. A simple and rapid determination of biapenem in plasma by high-performance liquid chromatography. Ikeda, K., Ikawa, K., Ikeda, A., Nishikawa, Y., Morikawa, N. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2006) [Pubmed]
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