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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review


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Disease relevance of Centrifugation


Psychiatry related information on Centrifugation


High impact information on Centrifugation


Chemical compound and disease context of Centrifugation

  • Tissue extracts were prepared by centrifugation of 10% homogenates at 100,000 X g for 30 min, and enzyme activity was measured in the protein fractions obtained by 40 and 47% ammonium sulfate saturation of the supernatant fluids from livers and hepatomas, respectively [12].
  • Murine fibrosarcoma cells can be separated into subpopulations by centrifugation through 10 to 35% Renografin density gradients [13].
  • Interactions of DNA and the gene product D5 (gpD5) of bacteriophage T5, a DNA-binding protein that binds preferentially and cooperatively to double-stranded DNA, were analyzed by metrizamide gradient centrifugation [14].
  • Shigella cytotoxin was purified from the culture medium of Shigella shigae 60 R and from the bacterial pellet by an extensive purification scheme involving chromatography on Cibachron Blue F3GA, chitin, and DEAE-cellulose columns, sucrose gradient centrifugation, and finally polyacrylamide gel electrophoresis under nondenaturing conditions [15].
  • The protein was overexpressed in Escherichia coli, and serine racemase activity was detected in the membrane but not in the cytoplasmic fraction after centrifugation of sonicated cells, whereas alanine racemase activity was located almost exclusively in the cytoplasm [16].

Biological context of Centrifugation

  • NDHU, NDHT, and methylnitrosourea [(MNU) CAS: 684-93-5; N-methyl-N-nitrosourea], a liver carcinogen only under special conditions, were tested for their ability, when injected ip into rats, to produce liver DNA damage measured as strand breaks by alkaline sucrose gradient centrifugation [17].
  • Phosphorylation of the receptor has been demonstrated by interaction of kinase 32P-phosphorylated proteins with anti-receptor antibody followed either by sucrose gradient centrifugation or SDS-PAGE of the immunoprecipitated proteins [18].
  • In an attempt to isolate the AVs, we subfractionated the ML suspension at different intervals after the induction of autophagy by VBL by centrifugation on a discontinuous Metrizamide gradient ranging from 50% to 15% [19].
  • The organizing center can be partially isolated by centrifugation of homogenates of metaphase eggs and will induce aster formation if mixed with tubulin from either activated or unactivated eggs [20].
  • It has been partially purified by sucrose gradient centrifugation and more extensively by diazobenzyloxymethyl-cellulose hybridization to plasmid DNA from a bacterial cDNA clone [21].

Anatomical context of Centrifugation


Associations of Centrifugation with chemical compounds

  • DNA was extracted from each population of mitotic cells and then was resolved into dense, BrdUrd-containing and light, unsubstituted DNA fractions by cesium chloride gradient centrifugation [27].
  • Using purified 32P-labeled RNA and 125I-labeled p12 protein, complexes that are stabilized by formaldehyde-cross-linking can be readily detected after velocity gradient centrifugation [28].
  • The defects of the mutants have been examined in heterozygous strains by native gel electrophoresis, glycerol gradient centrifugation, and immunoprecipitation [29].
  • The method involves rapid centrifugation of cells through a layer of silicone oil into perchloric acid after incubation periods as short as 4 s [30].
  • Comparison of the centrifugation behavior of PGE2 synthesis activity with that of various enzymes used as reference for the major subcellular entities has revealed that PGE2 synthesis fairly fits the density profile of sulfatase C in each case [31].

Gene context of Centrifugation


Analytical, diagnostic and therapeutic context of Centrifugation


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