Antibody array profiling reveals serum TSP-1 as a marker to distinguish benign from malignant prostatic disease.
BACKGROUND: Blood protein markers that provide more accurate or earlier diagnosis of prostate cancer should have a positive impact on prostate cancer treatment and management. METHODS: Serum samples from control subjects and patients with benign or malignant prostatic disease were analyzed on antibody arrays targeting multiple candidate prostate cancer markers and detected with two-color, rolling-circle amplification (TC-RCA). The measurements of certain antibodies were validated using immunoblots, immunoprecipitation/ mass spectrometry, and sandwich immunoassays. RESULTS: Several potential disease-associated protein alterations were uncovered. The most significant was thrombospondin-1, which was strongly elevated in patients with benign prostatic disease and repressed in patients with prostate cancer. Thrombospondin-1 levels did not correlate with prostate-specific antigen ( PSA) levels and differentiated benign from malignant disease with 79% sensitivity and 81% specificity. CONCLUSIONS: The measurement of thrombospondin-1 could be used to assist the decision to obtain a biopsy in men with suspected prostate cancer, which could lead to a reduction in the number of unnecessary prostatic biopsies. Prostate 67:255-267, 2007. (c) 2006 Wiley-Liss, Inc.[1]References
- Antibody array profiling reveals serum TSP-1 as a marker to distinguish benign from malignant prostatic disease. Shafer, M.W., Mangold, L., Partin, A.W., Haab, B.B. Prostate (2007) [Pubmed]
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