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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review


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Disease relevance of Immunoassay


Psychiatry related information on Immunoassay

  • Using a conformation-dependent immunoassay, it was further demonstrated that the PrP detected in the CSF from CJD patients was broadly similar in conformation to that found in non-CJD patients [6].
  • METHODS: ATPA, GAD65A, and GAD67A were determined (via a commercially available enzyme linked immunosorbent assay for ATPA, and a standardized radio immunoassays for GAD65A and GAD67A)in the sera of 239 patients with DSM-IV bipolar disorder, in 74 patients with DSM-IV schizophrenia, and in 220 healthy control subjects [7].
  • Hence, when the presence of the HRP marker of the immunoassay was determined using hydroquinone, the production of electroactive quinone was shown to permeate faster to the electrode, thus providing a faster response time [8].
  • S100B serum levels (quantitative immunoassay) and psychopathology (Positive and Negative Syndrome Scale, PANSS) were examined upon study admission and after 12 and 24 weeks of standardized treatment in 98 chronic schizophrenic patients with primarily negative symptoms [9].
  • New immunoassay for the mapping of neurofibrillary degeneration in Alzheimer's disease using two monoclonal antibodies against human paired helical filament tau proteins [10].

High impact information on Immunoassay

  • Do clinicians overestimate the diagnostic usefulness of parathyroid hormone immunoassays [11]?
  • C6 cells contain a replication-defective mutant T antigen that when tested in the DNA-binding immunoassay, showed no affinity for the ori fragment [12].
  • To determine whether somatostatin is found in the hypothalamus and extrahypothalamic brain, we studied autopsy brain tissue by specific immunoassay [13].
  • This immunoassay quantifies PrP isoforms by simultaneously following antibody binding to the denatured and native forms of a protein [14].
  • Such molecular probes will be helpful not only in standardizing immunoassays, but also in delineating the biological role of the various GH forms [15].

Chemical compound and disease context of Immunoassay


Biological context of Immunoassay


Anatomical context of Immunoassay


Associations of Immunoassay with chemical compounds

  • This material, which is urea stable, shows identical immunoassay dilution curves when measured with the highly specific K-30 glucagon antiserum [31].
  • MAIN OUTCOME MEASURES--Comparison of self-reported illicit drug use with results from urinary immunoassays for benzoylecgonine, a major cocaine metabolite [32].
  • Immunoassays were used to measure the levels of total testosterone, dihydrotestosterone (DHT), 3 alpha-androstanediol glucuronide (AAG), estradiol, SHBG, and prolactin in the stored (at -82 degrees C) plasma samples [33].
  • METHODS: Enzyme-linked immunoassay (EIA) was used to detect anti-p53 antibodies in 148 serum samples from 92 individuals occupationally exposed (in France or in Kentucky) to vinyl chloride; 15 of these individuals (six from France and nine from Kentucky) had ASL [34].
  • Yet, standard anticardiolipin antibodies (aCL) immunoassays routinely bind CL to microtiter wells by evaporation of the ethanol solvent overnight at 4 degrees C. Using a variety of techniques, we demonstrated that rapid oxidation occurs when CL is plated and exposed to air [35].

Gene context of Immunoassay

  • IL-10 protein was demonstrated by specific immunoassay and immunohistology [36].
  • Enzyme-linked immunoassay revealed 21.9 +/- 4.8 nM IL-8 in CF ELF compared with none in normals [37].
  • GDNF levels determined by immunoassay were higher in muscle than mucosal gut layers, and there was no difference between affected and unaffected segments of Hirschsprung's disease [38].
  • The mass of LCAT secreted, determined by immunoassay, did not differ in the native and mutant species [39].
  • Using gel shift immunoassays, we show that CDC2, cyclin A, and an RB-related protein are ubiquitous subunits of HiNF-D binding activity isolated from several cell types [40].

Analytical, diagnostic and therapeutic context of Immunoassay


  1. False-positive immunoassay for acetylcholine-receptor antibody in amyotrophic lateral sclerosis. Mittag, T.W., Caroscio, J. N. Engl. J. Med. (1980) [Pubmed]
  2. A bovine albumin peptide as a possible trigger of insulin-dependent diabetes mellitus. Karjalainen, J., Martin, J.M., Knip, M., Ilonen, J., Robinson, B.H., Savilahti, E., Akerblom, H.K., Dosch, H.M. N. Engl. J. Med. (1992) [Pubmed]
  3. Association of parvoviruses with rheumatoid arthritis of humans. Simpson, R.W., McGinty, L., Simon, L., Smith, C.A., Godzeski, C.W., Boyd, R.J. Science (1984) [Pubmed]
  4. Phosphatidyl choline is recognized by a series of Ly-1+ murine B cell lymphomas specific for erythrocyte membranes. Mercolino, T.J., Arnold, L.W., Haughton, G. J. Exp. Med. (1986) [Pubmed]
  5. Quantitation of cis-diamminedichloroplatinum II (cisplatin)-DNA-intrastrand adducts in testicular and ovarian cancer patients receiving cisplatin chemotherapy. Reed, E., Yuspa, S.H., Zwelling, L.A., Ozols, R.F., Poirier, M.C. J. Clin. Invest. (1986) [Pubmed]
  6. Absence of protease-resistant prion protein in the cerebrospinal fluid of Creutzfeldt-Jakob disease. Wong, B.S., Green, A.J., Li, R., Xie, Z., Pan, T., Liu, T., Chen, S.G., Gambetti, P., Sy, M.S. J. Pathol. (2001) [Pubmed]
  7. A high prevalence of organ-specific autoimmunity in patients with bipolar disorder. Padmos, R.C., Bekris, L., Knijff, E.M., Tiemeier, H., Kupka, R.W., Cohen, D., Nolen, W.A., Lernmark, A., Drexhage, H.A. Biol. Psychiatry (2004) [Pubmed]
  8. Construction of amperometric immunosensors based on the electrogeneration of a permeable biotinylated polypyrrole film. Ionescu, R.E., Gondran, C., Gheber, L.A., Cosnier, S., Marks, R.S. Anal. Chem. (2004) [Pubmed]
  9. S100B serum levels and long-term improvement of negative symptoms in patients with schizophrenia. Rothermundt, M., Ponath, G., Glaser, T., Hetzel, G., Arolt, V. Neuropsychopharmacology (2004) [Pubmed]
  10. New immunoassay for the mapping of neurofibrillary degeneration in Alzheimer's disease using two monoclonal antibodies against human paired helical filament tau proteins. Condamines, O., Buée-Scherrer, V., Boissier, L., Wattez, A., Delacourte, A., Pau, B., Mourton-Gilles, C. Neurosci. Lett. (1995) [Pubmed]
  11. Do clinicians overestimate the diagnostic usefulness of parathyroid hormone immunoassays? Minne, H.W., Weise, D., Ziegler, R. N. Engl. J. Med. (1984) [Pubmed]
  12. A small subclass of SV40 T antigen binds to the viral origin of replication. Scheller, A., Covey, L., Barnet, B., Prives, C. Cell (1982) [Pubmed]
  13. Somatostatin in human cerebrospinal fluid. Patel, Y., Rao, K., Reichlin, S. N. Engl. J. Med. (1977) [Pubmed]
  14. Eight prion strains have PrP(Sc) molecules with different conformations. Safar, J., Wille, H., Itri, V., Groth, D., Serban, H., Torchia, M., Cohen, F.E., Prusiner, S.B. Nat. Med. (1998) [Pubmed]
  15. Growth hormone heterogeneity: genes, isohormones, variants, and binding proteins. Baumann, G. Endocr. Rev. (1991) [Pubmed]
  16. Neutralisation of HIV isolates by anti-idiotypic antibodies which mimic the T4 (CD4) epitope: a potential AIDS vaccine. Dalgleish, A.G., Thomson, B.J., Chanh, T.C., Malkovsky, M., Kennedy, R.C. Lancet (1987) [Pubmed]
  17. An immunological determinant of RNase P protein is conserved between Escherichia coli and humans. Mamula, M.J., Baer, M., Craft, J., Altman, S. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  18. Quantitation of estrogen receptor in seventy-five specimens of breast cancer: comparison between an immunoassay (Abbott ER-EIA monoclonal) and a [3H]estradiol binding assay based on isoelectric focusing in polyacrylamide gel. Pousette, A., Gustafsson, S.A., Thörnblad, A.M., Nordgren, A., Sällström, J., Lindgren, A., Sundelin, P., Gustafsson, J.A. Cancer Res. (1986) [Pubmed]
  19. Hepatic fibrosis in rats produced by carbon tetrachloride and dimethylnitrosamine: observations suggesting immunoassays of serum for the 7S fragment of type IV collagen are a more sensitive index of liver damage than immunoassays for the NH2-terminal propeptide of type III procollagen. Ala-Kokko, L., Günzler, V., Hoek, J.B., Rubin, E., Prockop, D.J. Hepatology (1992) [Pubmed]
  20. Elevated serum D dimer: a degradation product of cross-linked fibrin (XDP) after intravenous streptokinase during acute myocardial infarction. Lew, A.S., Berberian, L., Cercek, B., Lee, S., Shah, P.K., Ganz, W. J. Am. Coll. Cardiol. (1986) [Pubmed]
  21. Expression cloning of a cDNA for human leukotriene C4 synthase, an integral membrane protein conjugating reduced glutathione to leukotriene A4. Lam, B.K., Penrose, J.F., Freeman, G.J., Austen, K.F. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  22. K+ channel blockers inhibit tissue factor expression by human monocytic cells. Crutchley, D.J., Conanan, L.B., Que, B.G. Circ. Res. (1995) [Pubmed]
  23. Replication of cloned DNA containing the Alu family sequence during cell extract-promoting simian virus 40 DNA synthesis. Ariga, H. Mol. Cell. Biol. (1984) [Pubmed]
  24. Prevalence and clinical significance of elevated antiphospholipid antibodies in patients with idiopathic thrombocytopenic purpura. Stasi, R., Stipa, E., Masi, M., Oliva, F., Sciarra, A., Perrotti, A., Olivieri, M., Zaccari, G., Gandolfo, G.M., Galli, M. Blood (1994) [Pubmed]
  25. SSPE, measles virus, and the matrix protein: report of a case with unusual immunochemical findings. Mandelbaum, D.E., Hall, W.W., Paneth, N., Wolff, R.R., DeVivo, D.C. Ann. Neurol. (1981) [Pubmed]
  26. Granulocyte/macrophage colony-stimulating factor and interleukin 3 release from human peripheral blood eosinophils and neutrophils. Kita, H., Ohnishi, T., Okubo, Y., Weiler, D., Abrams, J.S., Gleich, G.J. J. Exp. Med. (1991) [Pubmed]
  27. Enhanced interleukin-8 release and gene expression in macrophages after exposure to Mycobacterium tuberculosis and its components. Zhang, Y., Broser, M., Cohen, H., Bodkin, M., Law, K., Reibman, J., Rom, W.N. J. Clin. Invest. (1995) [Pubmed]
  28. The human lumbar intervertebral disc: evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, ageing, and degeneration. Antoniou, J., Steffen, T., Nelson, F., Winterbottom, N., Hollander, A.P., Poole, R.A., Aebi, M., Alini, M. J. Clin. Invest. (1996) [Pubmed]
  29. Diagnosis of aortic dissection by immunoassay for circulating smooth muscle myosin. Katoh, H., Suzuki, T., Hiroi, Y., Ohtaki, E., Suzuki, S., Yazaki, Y., Nagai, R. Lancet (1995) [Pubmed]
  30. Scrapie prion proteins accumulate in the cytoplasm of persistently infected cultured cells. Taraboulos, A., Serban, D., Prusiner, S.B. J. Cell Biol. (1990) [Pubmed]
  31. Salivary gland hyperglycemic factor: an extrapancreatic source of glucagon-like material. Lawrence, A.M., Tan, S., Hojvat, S., Kirsteins, L. Science (1977) [Pubmed]
  32. High prevalence of recent cocaine use and the unreliability of patient self-report in an inner-city walk-in clinic. McNagny, S.E., Parker, R.M. JAMA (1992) [Pubmed]
  33. Prospective study of sex hormone levels and risk of prostate cancer. Gann, P.H., Hennekens, C.H., Ma, J., Longcope, C., Stampfer, M.J. J. Natl. Cancer Inst. (1996) [Pubmed]
  34. Anti-p53 antibodies in sera of workers occupationally exposed to vinyl chloride. Trivers, G.E., Cawley, H.L., DeBenedetti, V.M., Hollstein, M., Marion, M.J., Bennett, W.P., Hoover, M.L., Prives, C.C., Tamburro, C.C., Harris, C.C. J. Natl. Cancer Inst. (1995) [Pubmed]
  35. Antiphospholipid antibodies are directed against epitopes of oxidized phospholipids. Recognition of cardiolipin by monoclonal antibodies to epitopes of oxidized low density lipoprotein. Hörkkö, S., Miller, E., Dudl, E., Reaven, P., Curtiss, L.K., Zvaifler, N.J., Terkeltaub, R., Pierangeli, S.S., Branch, D.W., Palinski, W., Witztum, J.L. J. Clin. Invest. (1996) [Pubmed]
  36. Immunoregulatory role of interleukin 10 in rheumatoid arthritis. Katsikis, P.D., Chu, C.Q., Brennan, F.M., Maini, R.N., Feldmann, M. J. Exp. Med. (1994) [Pubmed]
  37. Modulation of airway inflammation in cystic fibrosis. In vivo suppression of interleukin-8 levels on the respiratory epithelial surface by aerosolization of recombinant secretory leukoprotease inhibitor. McElvaney, N.G., Nakamura, H., Birrer, P., Hébert, C.A., Wong, W.L., Alphonso, M., Baker, J.B., Catalano, M.A., Crystal, R.G. J. Clin. Invest. (1992) [Pubmed]
  38. Glial-derived neurotrophic factor in human adult and fetal intestine and in Hirschsprung's disease. Bär, K.J., Facer, P., Williams, N.S., Tam, P.K., Anand, P. Gastroenterology (1997) [Pubmed]
  39. Effects of site-directed mutagenesis at residues cysteine-31 and cysteine-184 on lecithin-cholesterol acyltransferase activity. Francone, O.L., Fielding, C.J. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  40. Transcription of histone H4, H3, and H1 cell cycle genes: promoter factor HiNF-D contains CDC2, cyclin A, and an RB-related protein. van Wijnen, A.J., Aziz, F., Graña, X., De Luca, A., Desai, R.K., Jaarsveld, K., Last, T.J., Soprano, K., Giordano, A., Lian, J.B. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  41. Chromogranin A: its role in endocrine function and as an endocrine and neuroendocrine tumor marker. Deftos, L.J. Endocr. Rev. (1991) [Pubmed]
  42. Identification, culture, and characterization of pancreatic stellate cells in rats and humans. Bachem, M.G., Schneider, E., Gross, H., Weidenbach, H., Schmid, R.M., Menke, A., Siech, M., Beger, H., Grünert, A., Adler, G. Gastroenterology (1998) [Pubmed]
  43. The regulation of proenkephalin expression in a distinct population of glial cells. Melner, M.H., Low, K.G., Allen, R.G., Nielsen, C.P., Young, S.L., Saneto, R.P. EMBO J. (1990) [Pubmed]
  44. Influence of digoxin immune Fab therapy and renal dysfunction on the disposition of total and free digoxin. Ujhelyi, M.R., Robert, S., Cummings, D.M., Colucci, R.D., Green, P.J., Sailstad, J., Vlasses, P.H., Zarowitz, B.J. Ann. Intern. Med. (1993) [Pubmed]
  45. Measuring prions causing bovine spongiform encephalopathy or chronic wasting disease by immunoassays and transgenic mice. Safar, J.G., Scott, M., Monaghan, J., Deering, C., Didorenko, S., Vergara, J., Ball, H., Legname, G., Leclerc, E., Solforosi, L., Serban, H., Groth, D., Burton, D.R., Prusiner, S.B., Williamson, R.A. Nat. Biotechnol. (2002) [Pubmed]
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