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Tanaka, K. et al.

Ken Tanaka, Yosiaki Kuba, Tetsuro Sasaki, Fumiko Hiwatashi, Katsuko Komatsu,

Quantitation of curcuminoids in curcuma rhizome by near-infrared spectroscopic analysis.

This study investigated a nondestructive and rapid quantitation method for the curcuminoids, including curcumin, demethoxycurcumin, and bisdemethoxycurcumin, present in turmeric using near-infrared (NIR) spectroscopy and multivariate statistics. In the second derivatives of the NIR spectra of turmeric samples, two characteristic absorptions of curcuminoids were detected around 1700 and 2300-2320 nm. Partial least-squares regression (PLS-R) analysis was applied to the NIR spectra obtained from 34 turmeric samples, and PLS models for the quantitation of curcumin, demethoxycurcumin, bisdemethoxycurcumin, and total curcuminoid contents in the pulverized turmeric samples were constructed. Combination usage of the Standard Normal Variate (SNV) and second derivatives was obviously superior to other preprocessing methods. The lowest root mean squared error of cross-validation (RMSECV) values were detected at 6, 6, 6, and 6 PLS factors, for the quantitative subjects curcumin, demethoxycurcumin, bisdemethoxycurcumin, and total curcuminoid contents. It was clarified that the prediction of the composition by PLS-R analysis showed high correlation with the results of HPLC quantitations.[1]

References

Quantitation of curcuminoids in curcuma rhizome by near-infrared spectroscopic analysis. Tanaka, K., Kuba, Y., Sasaki, T., Hiwatashi, F., Komatsu, K. J. Agric. Food Chem. (2008) [Pubmed]

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