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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Isolation and characterisation of a bovine cDNA encoding eukaryotic initiation factor 2 alpha.

Two cDNA clones have been isolated, from a bovine lymphosarcoma library, that encode the alpha-subunit of eukaryotic initiation factor 2 (eIF-2 alpha). The predicted 315 amino acid sequence showed more than 99% amino acid identity with rat and human eIF-2 alpha. Galactose-regulated expression of a full length bovine eIF-2 alpha cDNA in yeast resulted in the synthesis of a polypeptide of the predicted molecular mass (36 kDa). Furthermore, the expressed polypeptide cross-reacted with an antibody raised against rabbit eIF-2 alpha confirming the identity of the cDNA.[1]

References

  1. Isolation and characterisation of a bovine cDNA encoding eukaryotic initiation factor 2 alpha. Green, S.R., Fullekrug, J., Sauer, K., Tuite, M.F. Biochim. Biophys. Acta (1991) [Pubmed]
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