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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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High-performance liquid chromatographic determination of histamine N-methyltransferase activity.

A method for the determination of histamine N-methyltransferase ( HMT) activity by high-performance liquid chromatography based on post-column derivatization with omicron-phthalaldehyde is described. The determination involves the separation of the substrate, histamine, from its product. N tau-methylhistamine, using a weak cation exchanger, followed by on-line derivatization of these imidazoleamines with omicron-phthalaldehyde and their detection and quantitation with a fluorimetric detector. This assay method is suitable for the measurement of HMT activity during enzyme purification.[1]

References

  1. High-performance liquid chromatographic determination of histamine N-methyltransferase activity. Fukuda, H., Yamatodani, A., Imamura, I., Maeyama, K., Watanabe, T., Wada, H. J. Chromatogr. (1991) [Pubmed]
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