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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Cloning and mapping of the potato virus Y genome and its in vitro expression.

Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein.[1]


  1. Cloning and mapping of the potato virus Y genome and its in vitro expression. Karchi, M., Sela, I., Edelbaum, O., Ori, A., Raccah, B., Rosner, A., Stram, Y. Virus Genes (1990) [Pubmed]
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