MicroRNA-profiling in formalin-fixed paraffin-embedded specimens.
The discovery of small regulatory RNA molecules during the last few years has changed our understanding of many biological and pathological processes. The most prominent and best analyzed class of these small regulatory noncoding RNAs is comprised by the microRNAs. The analysis of microRNA expression patterns is now widely used in biology and pathology employing a range of methodologies. However, many precious human tissue samples are only available as formalin-fixed paraffin-embedded (FFPE) specimen. In this chapter, the extraction of RNA from FFPE samples and the subsequent microRNA profiling utilizing fluorescence-labeled bead technology from Luminex Inc. is described.[1]References
- MicroRNA-profiling in formalin-fixed paraffin-embedded specimens. Lehmann, U. Methods Mol. Biol. (2010) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
