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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Nuclear transport of proteins translated in vitro from SP6 plasmid-generated mRNAs.

A sensitive and versatile assay is described for the nuclear transport of 35S-labeled proteins obtained by the in vitro translation of SP6 plasmid-generated mRNAs. A specific nuclear accumulation of greater than 20-fold is observed for the transformation-related nuclear proteins, p53 and E1b, and the nuclear enzyme, thymidine kinase, whereas transport of the nonnuclear proteins, dihydrofolate reductase and simian virus 40 small t antigen, is negligible within 30 min.[1]


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